The first thing we must keep in mind that one micro-RNA can only be used for diagnostics if it getting overexpressed or or underexpressed only in cancer tissue and not in normal one. But in your case as I can understand you have a single micro-RNA, with one micro-RNA from one data-set its difficult to access its diagnostic relevance. In such cases important aspect is to look for its disease association, that how the micro-rna is associated to the disease. If it is expressing then it must have some role in any of the hallmarks of cancer. Many times a kind of micro-RNA signature is predictive of a cancer stage or kind of disease, e.g. many microRNA signatures are distinct for metastatic and non-metastatic cancers. So you need to dig more before declaring it of a diagnostic relevance.
The first thing we must keep in mind that one micro-RNA can only be used for diagnostics if it getting overexpressed or or underexpressed only in cancer tissue and not in normal one. But in your case as I can understand you have a single micro-RNA, with one micro-RNA from one data-set its difficult to access its diagnostic relevance. In such cases important aspect is to look for its disease association, that how the micro-rna is associated to the disease. If it is expressing then it must have some role in any of the hallmarks of cancer. Many times a kind of micro-RNA signature is predictive of a cancer stage or kind of disease, e.g. many microRNA signatures are distinct for metastatic and non-metastatic cancers. So you need to dig more before declaring it of a diagnostic relevance.
Thank you for your Answer... One thing what i want to know, like i have one MicroRNA Signature which over express in both the cancer, so if we Diagnosed using RT PCR, so can we use the same microRNA probe for diagnosis of cancer patients..?
I am not getting you question, as you above said "both the cancer", does it mean in two different cancers or two cancer samples?
Rest, You can use same primer for detecting the presence of micro-RNA in patients as well. As i dont have any idea about your experiment so cant comment on the diagnostic applications.
Its seems premature to use this miRNA as a diagnostic marker, but it may be a good starting point for further studies.
I have to agree with one of the answers: I have trouble understanding the question. You are talking about two types of cancers (SCC and non-SCC in the same tissue for example), two different locations (oral and cervix for example), or just completely different cancers (SCC and glioblastoma for example)?
Next, I have to agree with a set of answers that diagnostic use may be still a stretch. Are you talking about serum, blood samples, or any other non-invasive or low invasive test (source of RNA)?
I am sure you can find a signature for SCCs from all types of tissues (mir-205, mir-31, perhaps mir-203 etc). But what does this signature help? Could you use it to diagnose-from serum- before most other, especially invasive test, will work?
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