Hello everyone,

I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of cells after fixation (PFA 4%), so I cannot continue with the staining in order to count them. I have tried to be very careful with the PBS washes between fixation and staining but each time I lose a good amount of cells. I would like your opinion if any of you have experienced the same problem.

Thanks a lot.