Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Why in BET surface area becomes zero but when detag ads des, it shows good value around 150-200? For single crystals is it a good data to publish?

For single crystals can I give BET data in the publication if it is coming around 200. And why it shows zero value surface area but after detag ads or des it shows value, and which one I should...

02 December 2020 7,173 1 View

How to analyse tga curve, when weight loss is not matching with constituents present in structure?

In TGA curve I am getting 2 step decomposition in which the 1st one coming around 185 to 195 degree centigrade. The weight loss calculated is not matching with the constituent present. Its a metal...

20 November 2020 714 5 View

Two different gaussian width in QM/MM Metadynamics?

Is it wise to use two different gaussians for two CVs during QM/MM metadynamics simulations?. Example for 0.5kcal/mol Gaussian height, For CV1 width is 0.3 and CV2 is 0.15. Where both CVs address...

10 March 2020 5,287 3 View

What is the impact of correlation test in Image encryption ?

Is correlation test sufficient for proving the efficiency of the encryption scheme?

23 February 2020 3,524 4 View

What is the significance of image entropy ( plain image and cipher image) in image processing?

Whether increase or decrease in entropy of cipher image is good for encryption? How?Why?

06 May 2019 9,299 12 View

Can PCR master mix solution be prepared in advance and stored at -20C?

For doing PCR, instead of preparing the PCR mater mix solution each and every time, is it advisable to prepare it in a larger quantity and store it at -20C so that the PCR can be set up faster....

27 January 2019 7,112 19 View

What are the other options to play with audio files apart from shifting and transformation?

Any other specific operations with speech files?

23 September 2018 9,243 2 View

Can I stain cells with Annexin V after fixing with 70% ethanol?

I have treated cancer cells with inhibitors of cell cycle. In addition to looking at the stage of cell cycle they are in, I want to see if there is any apoptosis induced by the treatment. But I...

28 July 2018 1,242 3 View

How to measure the mesh size in COMSOL for PCF design for FEM analysis?

I want to know how to measure mesh size in COMSOL when are using initializing mesh than refined mode? Is that possible?

28 February 2018 4,418 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View