How to convert HIV Copies/mL to IU/mL ?

I am developing HIV Viral Load Detection Assay, in that my Quantitative Standards are in Copies/mL, but I want to convert it to International Unit (IU)/mL as WHO recommendation to give results in...

08 September 2019 2,928 4 View

Problem in HIV RT PCR, every samples and water also coming positive at same time. Can anyone suggest what is the problem.?

I am using primer & Probe concentration 10 uM and using 0.5 uL in 25 uL reaction PCR setup. I have used so many different water but each water coming positive. Its might be primer dimer but...

01 February 2019 4,049 11 View

Which stabilizer we can used to stabilized RT PCR Master Mix?

I have developed RT PCR mastermix , now its required to add stabilizer in it? if yes which stabilizer we can use at which concentration.

11 December 2018 4,533 10 View

Which are the suitable gene target for DNA Extraction Internal control for RT PCR of DNA Virus?

We want Internal Control for DNA RT PCR, which should be act as a DNA extraction control.

05 June 2018 3,586 4 View

What is the best way to design DNA/RNA Extraction control for RT PCR testing?

I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.

03 April 2018 5,148 4 View

I am not getting Perfect Sigmoidal curve in RT PCR. Can any one suggest the Possible reasons?

Please suggest possible modifications in experiments.

10 November 2017 221 6 View

Can any one know stabilizer for PCR Master Mix?

I want to stabilize the RT PCR Master Mix at Room Temperature . Please suggest if any one having same experience or some stabilizer i can try.

09 October 2017 4,404 3 View

What is the best way to detect MicroRNA , Sybr Green or Taqman?

10 November 2015 821 8 View

What blocking buffers can I use for a Lateral Flow Immunoassay?

What blocking buffers can I use for a Lateral Flow Immunoassay? (NCM Blocking, after spraying)

06 July 2015 6,638 2 View

Problem in Real Time PCR?

31 December 2014 5,245 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

ANCOVA controlling baseline in RCT - how to perform power analysis and sample size estimation?

Hi, I have a simple question. I am hoping to perform a power analysis/sample size estimation for an RCT. We will be controlling for baseline symptoms, and using post-treatment or change scores as...

19 February 2021 2,648 23 View

MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View

How to get better phase shift in C-V curves for ferroelectric devices?

I tried to sweep my ferroelectric devices from -2V to 2V and then from 2V to -2V and The two hysteresis peaks are off by several microFarads. Should i increase bias voltage? I am doing these...

16 February 2021 8,514 2 View

Would you please recommend a function in R software that could be used with FUNGuild output to merge different rows that have similar guilds?

Dear colleagues, I have an output of FUNGuild tool (ASVs abundance in different samples and assigned to guilds) and would like to merge the ASVs that had similar ecological guilds using R...

16 February 2021 4,292 7 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View