Maulik Rachh

37 Questions 94 Answers 0 Followers

Questions related from Maulik Rachh

How to convert HIV Copies/mL to IU/mL ?

I am developing HIV Viral Load Detection Assay, in that my Quantitative Standards are in Copies/mL, but I want to convert it to International Unit (IU)/mL as WHO recommendation to give results in...

09 September 2019 3,478 4 View

Problem in HIV RT PCR, every samples and water also coming positive at same time. Can anyone suggest what is the problem.?

I am using primer & Probe concentration 10 uM and using 0.5 uL in 25 uL reaction PCR setup. I have used so many different water but each water coming positive. Its might be primer dimer but...

02 February 2019 4,130 11 View

Which stabilizer we can used to stabilized RT PCR Master Mix?

I have developed RT PCR mastermix , now its required to add stabilizer in it? if yes which stabilizer we can use at which concentration.

12 December 2018 4,719 10 View

Which are the suitable gene target for DNA Extraction Internal control for RT PCR of DNA Virus?

We want Internal Control for DNA RT PCR, which should be act as a DNA extraction control.

06 June 2018 3,664 4 View

What is the best way to design DNA/RNA Extraction control for RT PCR testing?

I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.

04 April 2018 5,235 4 View

I am not getting Perfect Sigmoidal curve in RT PCR. Can any one suggest the Possible reasons?

Please suggest possible modifications in experiments.

11 November 2017 327 6 View

Can any one know stabilizer for PCR Master Mix?

I want to stabilize the RT PCR Master Mix at Room Temperature . Please suggest if any one having same experience or some stabilizer i can try.

10 October 2017 4,511 3 View

What is the best way to detect MicroRNA , Sybr Green or Taqman?

Which method is best for microRNA detection.

11 November 2015 887 8 View

What blocking buffers can I use for a Lateral Flow Immunoassay?

What blocking buffers can I use for a Lateral Flow Immunoassay? (NCM Blocking, after spraying)

07 July 2015 6,745 2 View

Problem in R for generating Heatmap.

i am getting following error in R while generating heatmap. Error in .External.graphics(C_layout, num.rows, num.cols, mat, as.integer(num.figures), : invalid graphics state Please Guide me.

02 February 2015 8,166 3 View

Problem in Real Time PCR?

I optimised RT PCR, and its working very good, but suddenly i lost getting all the results, even positive control also. Please guide me the problem.

01 January 2015 5,324 4 View

Can anyone Provide me the protocol for Real Time PCR for MicroRNA Amplification and detection?

I want to perform RT PCR for MicroRNA Identification. Please provide me the protocol for the same.

12 December 2014 7,435 2 View

Is TaqMan a patented technology?

If we use a TaqMan probe for diagnostic purposes, is a licence required? If yes, which company do the patents belong to?

11 November 2014 6,625 8 View

Is anyone familiar with real time PCR of m tuberculosis.?

I am getting false positive results after 35 cycles, when my positive samples come at around the 20th cycle. How can I optimize these kinds of problems?

11 November 2014 5,604 2 View

Which is the best methods for RNA isolation from Breast Cancer Tissue?

I want to perform RT PCR for microRNA identification for Breast Cancer.

11 November 2014 3,379 6 View

How much primer and probe concentration are required for optimization of RT PCR for tuberculosis?

I am working on diagnosis of tuberculosis with Taqman Probe in RT PCR. But sometimes negative also comes positive & sometimes positive also comes negative. I am using Primer & Probe...

10 October 2014 6,740 2 View

Is 18s rRNA is right option for internal Control in RT PCR?

I am working on RT PCR for Tuberculosis. I want internal amplification control for my RT PCR. 18s rRNA is one of the house keeping gene. Can we use the same for PCR internal control ? Can it also...

09 September 2014 5,088 4 View

Can we use hsa-mir-199b for diagnosis of breast cancer using qRT-PCR?

hsa-mir-199b is overexpress microRNA for breast cancer, having fold change ratio 9.45. Can we use the same for the diagnosis of cancer patient?

09 September 2014 527 2 View

Is DEPC water important or can we use Milli Q water for RNA isolation?

If we used Milli Q with BioPak purification water in place of DEPC, can it work or it will affect the RNA purity ?

09 September 2014 1,445 12 View

How can I optimize real time PCR?

Can any one help me to describe step by step process?

08 August 2014 468 3 View

Does anyone have experience with DNA Isolation from sputum samples?

Ratio of 260/280 ~ 1.80, is really much matter for PCR or RT PCR ? Or ~2.00 will also works for the same.

08 August 2014 8,448 4 View

What is the importance of MicroRNA MicroArray profiling?

If we analysed microRNA microarray profiling, what that study suggest outcome of the data? Is its limited to Down & Up regulation of microRNA or its suggest something more?

08 August 2014 7,361 20 View

How to design primers for MicroRNA identification?

I want to perform Real Time PCR for MicroRNA identification study.

07 July 2014 2,134 2 View

Are intronic microRNAs a reaction mechanism or a defence mechanism of the body?

If microRNA comes from intervening sequences, its reaction of any mechanism or its itself generated body defence mechanism? Means its reaction of body process in disease creation stage or cellular...

07 July 2014 2,755 5 View

Why is there no amplification in Real Time PCR?

I am performing RT PCR for detection of tuberculosis. I am using Taqman Probe. I am not getting amplification. There is no curve line which cross the threshold line even in a positive sample.

04 April 2014 4,645 18 View

How do you prepare in-house Bst Polymerase?

Useful in LAMP Reaction.

04 April 2014 3,651 1 View

Why am I getting false positive result in -ve when working with LAMP?

I am working with LAMP (Loop-Mediated Isothermal Amplification) technology for the diagnosis of Tuberculosis. Why am I getting false positive result in -ve? We tried all new buffers and primers...

03 March 2014 702 16 View

Can anyone provide some information about MicroRNA thermodynamics?

The role of thermodynamics in MicroRNA prediction or designing?

03 March 2014 9,459 1 View

What is the ideal gene for internal control in real-time PCR?

Internal Control for real-time PCR required for test validation.

10 October 2013 6,527 18 View

Which is the best protocol for MicroRNA analysis using northern blotting with out commercial kits?

Reagents or buffer which are prepared in lab not any commercial reagents or kits.

10 October 2013 7,798 1 View

How to remove RNA isolation contamination?

09 September 2013 1,586 0 View

Can anyone suggest a RNA extraction method from tissue without the use of liquid nitrogen?

04 April 2013 1,620 9 View

It is possible that one MicroRNA is Upregulated for one Cancer and same MicroRNA is also Down regulated for the Same Cancer? If yes then Why?

04 April 2013 9,107 7 View

If we found same MicroRNA Expressing for Two Different Cancers, Can we use the same MicroRNA for Diagnosis of Both the Cancer?

04 April 2013 996 7 View

How to generate MicroRNA Heatmap?

04 April 2013 9,754 2 View

What is the meaning of down regulated microRNA?

Cancer microRNA.

03 March 2013 7,657 11 View

Which is the best protocol for Antibody Gold Conjugation?

Gold Conjugation Preparation Protocol

01 January 1970 606 6 View