37 Questions 94 Answers 0 Followers
Questions related from Maulik Rachh
I am developing HIV Viral Load Detection Assay, in that my Quantitative Standards are in Copies/mL, but I want to convert it to International Unit (IU)/mL as WHO recommendation to give results in...
09 September 2019 3,530 4 View
I am using primer & Probe concentration 10 uM and using 0.5 uL in 25 uL reaction PCR setup. I have used so many different water but each water coming positive. Its might be primer dimer but...
02 February 2019 4,145 11 View
I have developed RT PCR mastermix , now its required to add stabilizer in it? if yes which stabilizer we can use at which concentration.
12 December 2018 4,805 10 View
We want Internal Control for DNA RT PCR, which should be act as a DNA extraction control.
06 June 2018 3,684 4 View
I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.
04 April 2018 5,254 4 View
Please suggest possible modifications in experiments.
11 November 2017 342 6 View
I want to stabilize the RT PCR Master Mix at Room Temperature . Please suggest if any one having same experience or some stabilizer i can try.
10 October 2017 4,542 3 View
Which method is best for microRNA detection.
11 November 2015 905 8 View
What blocking buffers can I use for a Lateral Flow Immunoassay? (NCM Blocking, after spraying)
07 July 2015 6,771 2 View
i am getting following error in R while generating heatmap. Error in .External.graphics(C_layout, num.rows, num.cols, mat, as.integer(num.figures), : invalid graphics state Please Guide me.
02 February 2015 8,189 3 View
I optimised RT PCR, and its working very good, but suddenly i lost getting all the results, even positive control also. Please guide me the problem.
01 January 2015 5,338 4 View
I want to perform RT PCR for MicroRNA Identification. Please provide me the protocol for the same.
12 December 2014 7,446 2 View
If we use a TaqMan probe for diagnostic purposes, is a licence required? If yes, which company do the patents belong to?
11 November 2014 6,646 8 View
I am getting false positive results after 35 cycles, when my positive samples come at around the 20th cycle. How can I optimize these kinds of problems?
11 November 2014 5,615 2 View
I want to perform RT PCR for microRNA identification for Breast Cancer.
11 November 2014 3,391 6 View
I am working on diagnosis of tuberculosis with Taqman Probe in RT PCR. But sometimes negative also comes positive & sometimes positive also comes negative. I am using Primer & Probe...
10 October 2014 6,754 2 View
I am working on RT PCR for Tuberculosis. I want internal amplification control for my RT PCR. 18s rRNA is one of the house keeping gene. Can we use the same for PCR internal control ? Can it also...
09 September 2014 5,102 4 View
hsa-mir-199b is overexpress microRNA for breast cancer, having fold change ratio 9.45. Can we use the same for the diagnosis of cancer patient?
09 September 2014 540 2 View
If we used Milli Q with BioPak purification water in place of DEPC, can it work or it will affect the RNA purity ?
09 September 2014 1,459 12 View
Can any one help me to describe step by step process?
08 August 2014 479 3 View
Ratio of 260/280 ~ 1.80, is really much matter for PCR or RT PCR ? Or ~2.00 will also works for the same.
08 August 2014 8,460 4 View
If we analysed microRNA microarray profiling, what that study suggest outcome of the data? Is its limited to Down & Up regulation of microRNA or its suggest something more?
08 August 2014 7,374 20 View
I want to perform Real Time PCR for MicroRNA identification study.
07 July 2014 2,145 2 View
If microRNA comes from intervening sequences, its reaction of any mechanism or its itself generated body defence mechanism? Means its reaction of body process in disease creation stage or cellular...
07 July 2014 2,766 5 View
I am performing RT PCR for detection of tuberculosis. I am using Taqman Probe. I am not getting amplification. There is no curve line which cross the threshold line even in a positive sample.
04 April 2014 4,656 18 View
Useful in LAMP Reaction.
04 April 2014 3,662 1 View
I am working with LAMP (Loop-Mediated Isothermal Amplification) technology for the diagnosis of Tuberculosis. Why am I getting false positive result in -ve? We tried all new buffers and primers...
03 March 2014 720 16 View
The role of thermodynamics in MicroRNA prediction or designing?
03 March 2014 9,471 1 View
Internal Control for real-time PCR required for test validation.
10 October 2013 6,538 18 View
Reagents or buffer which are prepared in lab not any commercial reagents or kits.
10 October 2013 7,809 1 View
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09 September 2013 1,598 0 View
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04 April 2013 1,631 9 View
04 April 2013 9,118 7 View
04 April 2013 1,011 7 View
04 April 2013 9,765 2 View
Cancer microRNA.
03 March 2013 7,669 11 View
Gold Conjugation Preparation Protocol
01 January 1970 617 6 View
