I am trying to run IP protein from crosslinked (DSP) cell lysate in native gel. I am getting a smear in ATGL IP on native gel ?

17 August 2019 0 5K Report

I have crosslinked the 3T3L1 cell using DSP and IP ATGL and MTTP proteins using AG beads. when I run the ATGL IP in native gel I am getting a smear. Where as my MTTP IP works fine. Please give your suggestion and comments to overcome this problem. Also suggest what should use as ladder for native gel? Till now I was using commercially available ladder for native gel, but I was wondering since commercially available ladder will have SDS it wont be giving accurate molecular weight.

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