Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine....
In our lab, we dilute the 10X running buffer to 1X and reuse it several time. So I wondered if this could be the cause. I tried a fresh run with a freshly diluted 1X buffer and replaced the Acrylamid, 10% SDS, APS, and TEMED, but still no bands at all.
I don't think it's a sample-related issue because I'm using a protein marker that I just bought to check the bands.
Any suggestions on how to resolve the following condition (the gel is torn, but even if it's an intact gel, I can't see the bands like that)?
Hyunjin Kim SDS PAGE is for Protein separation, there may be no proteins in your sample
The fact that you can't see the bands from your protein standards means the issue isn't specific to your samples.
This might be a dumb question, but did you see the loading dye and/or the colored standards move through the gel? My first guess is that there is something wrong with the electronics of your gel rig.
Lenin Kumar Bompalli the issue is that Hyunjin Kim can't detect any proteins. They loaded a protein standard as a marker & can't see it either.
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