I am trying to resolve immune cytokines. Which is the right percentage of SDS resolving gel?

26 April 2024 0 10K Report

I wish to resolve IL-6 (mol wt= 21-26KDa), TNF- alpha (17.3-17.4 KDa), GMF-beta (16.7 KDa), GAPDH (36kDa) and PDL1 (40-70 KDa). I think 12.5% gel is good to go. Is it advisable or should I go for lesser percentages?

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