Cannot open big files in GraphPad Prism?

Good morning. I have recently switched computers and gotten a new edition of Graphpad. I am trying to open a very large file containing all of my graphs for a project, but am encountering an...

22 March 2020 6,810 1 View

Sorting negative cells from GFP dim parental, please help?

I have an established cell line that expresses GFP constitutively. Once I transfect the cell line, there shouldn't any GFP expression since the DNA payload doesn't have a fluorescence marker. My...

23 September 2019 3,940 1 View

FACS-related Co-op project, can someone help me with their input?

Good evening - I need to come up with a project idea for co-op. The project needs to be mostly FACS-related but also give exposure to other techniques, such as cell culture and molecular biology...

18 September 2019 8,911 2 View

How do I run moderation with repeated samples and no experimental manipulation?

Hi everyone! I was wondering if anyone might have any ideas of how to conduct some potential analyses for some research I am working on. I am interested in the moderating role of trait emotion...

09 May 2019 5,118 3 View

How to link SAP2000 to matlab?

About connecting SAP with matlab

16 November 2018 2,663 3 View

How to link opensees to matlab?

I want to link matlab to opensees to complete the dynamic stability analysis of a 2D frame

13 August 2018 3,721 4 View

I want to measure the level of the tlr4 in tumor tissue, which method is better Immunohistochemistry vs western blot?

Dear all, Anybody could explain to me which method is better for measurement the level of toll like receptor protein in tumor tissue , Immunohistochemistry vs western blot? Thanks in advance.

09 April 2018 6,343 5 View

What is the pH to reach in measuring titratable acidity?

Dear all, For measuring the titratable acidity, I see titration of sample with 0.1 N NaOH to reach specific pH and reported by lactic acid. But the specifi pH I see is different in literiture,...

15 March 2018 8,141 3 View

Any tips for rodent sperm isolation/RNA extraction from sperm?

My lab is interested in extracting RNA from sperm (small RNAs specifically), and I believe we have the correct kit (Zymo Quick-RNA Micro-prep), but we already have a lot of trouble with extracting...

06 August 2017 8,639 1 View

Measuring prebiotic activity of a fruit

05 August 2017 5,723 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How to analyse monosaccharide compositions from soluble fiber?

I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found...

17 February 2021 5,230 1 View

No title

Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Which non-coordinating solvent dissolves anhydrous Iron(II)chloride?

Hallo, i am looking for a non coordinating solvent that dissolves anhydrous FeCl2 for a synthesis in which i can not use solvents like thf, pyridin etc.. I already tried benzene (no solubility),...

11 February 2021 6,105 1 View