Does anyone know what might be causing the smeared bands on my western blot?

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Grace Lee

Subhajit Karmakar

Thank you so much for your suggestions. I will try decreasing the voltage and see if we can get better results :)

Yunyuan Li

I will suggest you to do enough blocking (fresh milk and longer)and reduce primary and secondary antibody concentrations. When you do ECL, You may also dry the membrane before imaging.

Yunyuan Li Thank you so much. I will try that very soon!