Hi, I am confused with the calculation regarding the BOD5 for seeded dilutions using the below formula

BOD mg/l = (D1-D2)-(S)Vs/P (As per standard methods).

Could any one of you please explain how this formula is applied in calculation where 10 ml of seed is added to 300 ml BOD bottle containing buffered dilution water (seeded control) and another bottle containing 5 ml of waste water sample and 5ml of seed in a 300 ml bottle (seeded sample). The initial and final DO being 8.6 and 5.8 mg/l for seeded control and 8.7 and 4.8 mg/l for the seeded sample respectively.