I am trying to run IP protein from crosslinked (DSP) cell lysate in native gel. I am getting a smear in ATGL IP on native gel ?

I have crosslinked the 3T3L1 cell using DSP and IP ATGL and MTTP proteins using AG beads. when I run the ATGL IP in native gel I am getting a smear. Where as my MTTP IP works fine. Please give...

17 August 2019 4,598 0 View