how can we determine through CD whether protein has been unfolded in presence of 8M urea or 6M guanidine HCL ?
How can we explain the unfolding of protein in presence of high concentrations of denaturants
one strand - SSDNA - BIOTIN LABELLED another strand - SSDNA - NO LABEL without using NaCl , for Bli experiment ?
27 November 2023 9,035 0 View
How to Distinguish between DH5 alpha and BL21 cells if they are not labelled ?
25 August 2023 7,001 0 View
can you plese suggest other ways of restriction digestion of DNA.
19 June 2023 7,900 3 View
How can the size of protein (6kda) , dna (18bp, app. 6kda size ) and resultant complex around 12kda effects the change in the Fluorescence anisotropy ? Is there any correlation between the size...
01 April 2023 894 1 View
I am working on SSR markers.I have used Genodive software for analysis of tetraploid data. To check the genetic differentiation in 12 populations I applied AMOVA and the result file has attached....
11 April 2022 7,186 2 View
I don't know what happened. Suddenly I am unable to find my previous runs (DLS and zetapotential) data in my dts file. Even .del file was also not created which means data was not deleted. Anybody...
02 February 2022 9,008 5 View
can we use a palindromic sequence having dsDNA labelled with cyanine fluorophores for fret experiments ?
05 October 2021 5,629 1 View
y = A1*exp(-x/t1) + A2*exp(-x/t2)+y0
14 July 2021 7,668 3 View
What are the recommended buffers and ionic strengths for pH 10 , pH 12, pH 14, to study protein secondary structure in circular dichroism ?
04 June 2021 7,942 4 View
Can any one suggest me concentration of beta-mercaptoethanol (2-ME) or DTT to prevent cysteine dimerisation in protein purification at pH 8.0 . and stability of them in Tris Hcl buffer with...
16 December 2019 3,160 4 View
Hi... I seem to be in a very big trouble. My His columns look cracked after my last purification. In another column, the materials seem to be pushed. How is it possible and is there anything I...
24 June 2024 7,071 2 View
Hello everyone, I'm currently having issues in trying to obtain an extracted DNA sample with 260/230 ratio values ranging from 2.0 to 2.2 for Nanopore downstream analysis. Right now after...
01 November 2023 5,609 0 View
Hello, I need to use guanidium chloride to denature a protein. However, I’m reluctant because it is absolutely crucial for my experiment that no isocyanic acid is formed. I need to know the...
13 September 2023 4,587 1 View
I am running mouse amyloid beta 42 ELISA using the kit from Invitrogen. According the manufacturers guideline, the samples should be diluted so that the concentration of guanidine in final...
07 September 2023 7,840 2 View
Came across two methods guanidine extraction protocol and ctab protocol. Which of these two will be most appropriate and error free?
01 August 2023 4,623 0 View
Hi, i wonder if i can sterillize guanidine thiocyanate 2M solution for qPCR purposes (to remove RNase and DNase), does anyone knows how the high temp impacts the solution? thanks
11 January 2023 2,880 2 View
The use of bleach to deactivate guanidine hydrochloride containing buffers is well documented to be hazardous. What is a suitable alternative disinfectant?
15 July 2022 4,228 0 View
I have been using Dyanebeads MyOne Silane beads to isolate both RNA and DNA. I get extremely low 260/230 ratio after each isolation. I am assuming it is caused by RLT buffer which contains...
02 August 2021 8,361 0 View
increase of DNA and RNA binding to spin column to highest capacity
11 July 2021 6,269 2 View
Hi My protein of interest is only expressed in E. Coli insoluble fraction. So, I tried to extract them adding 6 M guanidine to the insoluble pellet. By rotating the mixture of pellet and solution...
11 March 2021 1,578 3 View