How can the size of protein (6kda) , dna (18bp, app. 6kda size ) and resultant complex around 12kda effects the change in the Fluorescence anisotropy ? Is there any correlation between the size and change in anisotropy ?
Adam B Shapiro
Please see this document for the relationship between molecular weight, fluorescence lifetime and polarization.
https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/technical-notes-and-product-highlights/fluorescence-polarization-fp.html
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