What is the effect of size of the individual components and complex formed in DNA protein interactions effect the change in Fluorescence anisotropy ?

02 April 2023 1 894 Report

How can the size of protein (6kda) , dna (18bp, app. 6kda size ) and resultant complex around 12kda effects the change in the Fluorescence anisotropy ? Is there any correlation between the size and change in anisotropy ?

Adam B Shapiro

Please see this document for the relationship between molecular weight, fluorescence lifetime and polarization.

https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/technical-notes-and-product-highlights/fluorescence-polarization-fp.html

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