Kandluri Geethika

13 Questions 31 Answers 0 Followers

Questions related from Kandluri Geethika

Is there any simulation or computational tool to analyze possible conformations in presence of Denaturing agent like 8MUrea or 8Mguanidium chloride ??

09 July 2025 1,298 0 View

Can CHS (Cholesteryl Hemisuccinate) Tris salt effectively stabilize proteins in solution state at pH 7.5 ??

can any body suggest me any stabilizing agents to reduce protein aggregation and improve protein stability in solution at pH 7.5 ??

12 June 2025 760 1 View

How can I specifically biotinylate Proteins in either N terminal of C terminal when my protein is stable at pH 7.5can any body suggest me protocol ?

Stability of protein is at Ph 7.5 ? How can we control and only have site specific biotinylation ?

03 June 2025 8,178 1 View

How can we anneal biotin labelled ssdna and unlabelled ssdna to Ds DNA with around 70 percent GC and TM of around 80 degrees ?

one strand - SSDNA - BIOTIN LABELLED another strand - SSDNA - NO LABEL without using NaCl , for Bli experiment ?

28 November 2023 9,069 0 View

How to Distinguish between dh5 alpha and bl21 cells ?

How to Distinguish between DH5 alpha and BL21 cells if they are not labelled ?

26 August 2023 7,031 0 View

What is the effect of size of the individual components and complex formed in DNA protein interactions effect the change in Fluorescence anisotropy ?

How can the size of protein (6kda) , dna (18bp, app. 6kda size ) and resultant complex around 12kda effects the change in the Fluorescence anisotropy ? Is there any correlation between the size...

02 April 2023 927 1 View

How can we remove nucleic acid contamination from a DNA binding protein elute fractions ?

nearly pure fraction of DNA binding protein (around 6.6 kda) is contaminated with the Nucleic acid size of around 100 - 150 bp , How can we separate them and collect protein fraction intact?

16 March 2022 9,035 3 View

Can we use a palindromic sequence having dsDNA labelled with fluorophores for fret experiments ?

can we use a palindromic sequence having dsDNA labelled with cyanine fluorophores for fret experiments ?

06 October 2021 5,657 1 View

How to understand the goodness of fit in exponential curves to analyze fast kinetics ?

y = A1*exp(-x/t1) + A2*exp(-x/t2)+y0

15 July 2021 7,701 3 View

What are the recommended buffers and ionic strengths for pH 10 , pH 12, pH 14, to study protein in circular dichroism ?

What are the recommended buffers and ionic strengths for pH 10 , pH 12, pH 14, to study protein secondary structure in circular dichroism ?

05 June 2021 7,972 4 View

How can we determine through CD whether protein has been unfolded in presence of 8M urea or 6M guanidine HCL ?

how can we determine through CD whether protein has been unfolded in presence of 8M urea or 6M guanidine HCL ? How can we explain the unfolding of protein in presence of high concentrations of...

04 February 2021 7,447 0 View

Are there any proteins that can not be denatured by Urea or Guanidium HCL ?

Are there any proteins that can not be denatured by Urea or Guanidium HCL ? If SO can anyone give me an example ??

15 January 2021 2,349 1 View

Can any one suggest me concentration of beta-mercaptoethanol (2-ME) or DTT to prevent cysteine dimerisation in protein purification?

Can any one suggest me concentration of beta-mercaptoethanol (2-ME) or DTT to prevent cysteine dimerisation in protein purification at pH 8.0 . and stability of them in Tris Hcl buffer with...

17 December 2019 3,183 4 View