Kandluri Geethika

3 Questions 21 Answers 0 Followers

Questions related from Kandluri Geethika

How to understand the goodness of fit in exponential curves to analyze fast kinetics ?

y = A1*exp(-x/t1) + A2*exp(-x/t2)+y0

15 July 2021 7,638 3 View

What are the recommended buffers and ionic strengths for pH 10 , pH 12, pH 14, to study protein in circular dichroism ?

What are the recommended buffers and ionic strengths for pH 10 , pH 12, pH 14, to study protein secondary structure in circular dichroism ?

05 June 2021 7,910 4 View

Can any one suggest me concentration of beta-mercaptoethanol (2-ME) or DTT to prevent cysteine dimerisation in protein purification?

Can any one suggest me concentration of beta-mercaptoethanol (2-ME) or DTT to prevent cysteine dimerisation in protein purification at pH 8.0 . and stability of them in Tris Hcl buffer with...

17 December 2019 3,105 4 View