Kandluri Geethika

7 Questions 29 Answers 0 Followers

Questions related from Kandluri Geethika

How can we anneal biotin labelled ssdna and unlabelled ssdna to Ds DNA with around 70 percent GC and TM of around 80 degrees ?

one strand - SSDNA - BIOTIN LABELLED another strand - SSDNA - NO LABEL without using NaCl , for Bli experiment ?

28 November 2023 9,007 0 View

How to Distinguish between dh5 alpha and bl21 cells ?

How to Distinguish between DH5 alpha and BL21 cells if they are not labelled ?

26 August 2023 6,973 0 View

What is the effect of size of the individual components and complex formed in DNA protein interactions effect the change in Fluorescence anisotropy ?

How can the size of protein (6kda) , dna (18bp, app. 6kda size ) and resultant complex around 12kda effects the change in the Fluorescence anisotropy ? Is there any correlation between the size...

02 April 2023 866 1 View

Can we use a palindromic sequence having dsDNA labelled with fluorophores for fret experiments ?

can we use a palindromic sequence having dsDNA labelled with cyanine fluorophores for fret experiments ?

06 October 2021 5,604 1 View

How to understand the goodness of fit in exponential curves to analyze fast kinetics ?

y = A1*exp(-x/t1) + A2*exp(-x/t2)+y0

15 July 2021 7,645 3 View

What are the recommended buffers and ionic strengths for pH 10 , pH 12, pH 14, to study protein in circular dichroism ?

What are the recommended buffers and ionic strengths for pH 10 , pH 12, pH 14, to study protein secondary structure in circular dichroism ?

05 June 2021 7,919 4 View

Can any one suggest me concentration of beta-mercaptoethanol (2-ME) or DTT to prevent cysteine dimerisation in protein purification?

Can any one suggest me concentration of beta-mercaptoethanol (2-ME) or DTT to prevent cysteine dimerisation in protein purification at pH 8.0 . and stability of them in Tris Hcl buffer with...

17 December 2019 3,120 4 View