can you plese suggest other ways of restriction digestion of DNA.
are you seeing anything on the gel. The uncut dna or the size ladder?What type of dna are you cutting and how much dna are you cutting and loading on to the gel.
It will be helpful if you could share more details about the protocol & amounts of template/enzyme that you have used. Not being able to see any bands could be due to overdigestion of the template.
Have you checked the concentration and quality of uncut DNA before restriction digestion? Did it have sufficient concentration and its integrity was good? On gel, where you were checking digestion, did you use a DNA marker since it might help whether there is any issue during electrophoresis or not? If you cannot even see the marker on the gel, there must be something wrong with your gel or running buffer. If the DNA has good quality and concentration and there is no issue with the electrophoresis process you must get at least a band of backbone. I hope this will be helpful to you. Wish you the best!
- Badges
- Science method