one strand - SSDNA - BIOTIN LABELLED
another strand - SSDNA - NO LABEL
without using NaCl , for Bli experiment ?
How to Distinguish between DH5 alpha and BL21 cells if they are not labelled ?
25 August 2023 7,001 0 View
can you plese suggest other ways of restriction digestion of DNA.
19 June 2023 7,900 3 View
How can the size of protein (6kda) , dna (18bp, app. 6kda size ) and resultant complex around 12kda effects the change in the Fluorescence anisotropy ? Is there any correlation between the size...
01 April 2023 894 1 View
I am working on SSR markers.I have used Genodive software for analysis of tetraploid data. To check the genetic differentiation in 12 populations I applied AMOVA and the result file has attached....
11 April 2022 7,186 2 View
I don't know what happened. Suddenly I am unable to find my previous runs (DLS and zetapotential) data in my dts file. Even .del file was also not created which means data was not deleted. Anybody...
02 February 2022 9,008 5 View
can we use a palindromic sequence having dsDNA labelled with cyanine fluorophores for fret experiments ?
05 October 2021 5,629 1 View
y = A1*exp(-x/t1) + A2*exp(-x/t2)+y0
14 July 2021 7,668 3 View
What are the recommended buffers and ionic strengths for pH 10 , pH 12, pH 14, to study protein secondary structure in circular dichroism ?
04 June 2021 7,942 4 View
Can any one suggest me concentration of beta-mercaptoethanol (2-ME) or DTT to prevent cysteine dimerisation in protein purification at pH 8.0 . and stability of them in Tris Hcl buffer with...
16 December 2019 3,160 4 View
I have calculated the distance matrix in populations software. The data which i used is microsatellite data. I saved the distance matrix file in .txt format.But when I chose this same file to...
30 June 2019 2,763 6 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View