Autclaving guanidine thiocyanate 2M solution?

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What is the most effective protocol for extracting cells from breast cancer tissues? Which enzyme is considered ideal for this purpose?

I am currently conducting my PhD research aimed at isolating and culturing cells from breast cancer tissues for further molecular and cellular analyses. Effective cell extraction from tissue...

11 April 2024 4,381 4 View

Which is the Lipofectamine Transfection Protocol for plasmid DNA (PC3 Eb KO)?

Lipofectamine is a commonly used transfection reagent known for its efficiency in delivering nucleic acids into mammalian cells. However, the optimal transfection conditions can vary depending on...

11 April 2024 5,893 2 View

Prohibitions or limitations in adapting a scale into another language and culture?

Besides the procedures in adapting a psychological scale, are there any prohibitions or limitations in adapting a scale into another language and culture?

29 February 2024 8,875 3 View

Is it permissible to use contextual information within parentheses "()" when developing psychological items in adaptation scale?

Currently, I am adapting the Workforce Agility scale adapted to Indonesian language and culture. To provide further clarification for items, I am adding contextual information within parentheses...

29 February 2024 5,159 3 View

How to Calibrate Molecular Weight of Protein Emulsion Using Zetasizer Ultra?

Hello everyone, recently I have updated my Zetasizer software for molecular weight calibration. I have done calibration using Toluene HPLC grade 99% for the reference, but I encounter some issue...

19 July 2023 9,429 3 View

Rashba-like splitting at the VBM of Wannier band structure, but no splitting at the VBM of DFT band structure (same material). Any suggestions?

Dear all, I recently did DFT calculation and Wannier interpolation for the band structure of a janus monolayer (SOC was considered). In general, the obtained DFT band structure and Wannier band...

05 January 2023 9,837 4 View

Does everyone know how adapt microalgae Porphyridium cruentum from sea water media to fresh water media ?

I am having a research about Porphyridum cruentum, and i would like to adapt it from sea water media to fresh water media. If you have knowledge about it, I will be so appreciate if you would like...

25 June 2022 8,642 0 View

What tool do you use to run your safety risk assement?

Dear all researchers, I am a new researcher in ICT, I am an Instructor in Safety Management System training. One of our syllabi is accident scenario and risk assessment practice. Is there any...

20 June 2022 7,288 8 View

What is the appropriate Multilevel Reliability index?

Dear all, Hope you are safe. I would like to know what is the best reliability index to describe. I'm using Psych package and I assume that I am a low-intermediate level of R user. I conduct a...

05 November 2020 7,567 1 View

I need questionnaire sample related to implementation of new policy/regulation for covid-19?

Dear All Researcher, Please help me, I need a questionnaire sample to analyse the impact of implementation of new regulation related to covid-19 to the company/organization. I'm very thank you...

05 August 2020 5,066 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Size-biased recovery of total RNA isolated with Trizol vs RNeasy?

So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...

14 July 2024 641 1 View

Is DNase I (RNase-Free) necessary to be used before cDNA synthesis?

I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...

27 June 2024 2,951 5 View

Why does my protein bind poorly to the Ni sepharose beads?

I am purifying RNAse E in E. coli (118 kDa). I cloned the rne coding sequence on pET28a with a His tag at the N-terminus. The protein overexpressed fine (see attached figures). The binding buffer...

24 June 2024 6,287 4 View

His column crack?

Hi... I seem to be in a very big trouble. My His columns look cracked after my last purification. In another column, the materials seem to be pushed. How is it possible and is there anything I...

24 June 2024 7,071 2 View

Does autoclaving reduce the concentration of heavy metals in sewage wastewater?

I'm currently studying the treatment of sewage wastewater and am interested in understanding the impact of autoclaving on the concentration of heavy metals. Specifically, I would like to know if...

10 June 2024 2,579 4 View

Can I use DNase I and RNase R in the same moment?

Hi, I want to obtain circular RNA and lariat. I need treat my samples with DNase I and RNase R. Do you know, I can do this in the same time? And maybe do you have any protocols for simultaneous...

02 June 2024 9,544 0 View

DNA foot-printing buffer components? What are their roles?

Hi, I am learning to perform footprinting experiment. In the protocol I have there are the following components of the reaction buffer: HEPES buffer (7.8 pH), glycerol, BSA, DTT, KCl, MgCl2,...

28 May 2024 1,317 0 View

Is it necessary to sterilize soil samples before preparing contaminated soil for experiments on degradation of organic pollutants in soil by PMS?

Is it necessary to sterilize soil samples before preparing contaminated soil for experiments on degradation of organic pollutants in soil by PMS? A review of the large amount of papers shows that...

24 April 2024 1,417 2 View