Came across two methods guanidine extraction protocol and ctab protocol. Which of these two will be most appropriate and error free?
Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...
30 July 2024 3,849 0 View
through origin software
17 July 2024 4,991 0 View
As part of my thesis I'll be conducting a single-sample pre-post intervention analysis, in which my only DV is ordinal (7-point Likert scale). In my research proposal I said that I would use...
05 June 2024 3,122 2 View
Snowball metrics is used by scival.com to report some performance indicators. This metrics seems rather obscure from the mathematical standpoint. Unfortunately there is apparently (after a quick...
05 June 2024 7,463 0 View
I carried out a normality test on my DV and turns out it is significant hence violating the assumption. Do I carry on with multiple regression or what else can be done in this case?
30 May 2024 963 2 View
Tree growth is an important aspect of forestry and forest ecology. Typically a growth rate is calculated as the difference between two stem diameter measurements over a given time interval: (dbh2...
15 May 2024 8,739 2 View
How do you think technology can help hospitalized children?
10 May 2024 950 2 View
Someone has a computer program, algorithm or spreadsheet to solve problems of transients on a water flow in open channel regime through a circular section (flowing inside a pipe with free surface)?
10 April 2024 9,477 2 View
What AI weaponry means to you? what are the types?
06 April 2024 3,384 1 View
I recently performed Illumina shotgun sequencing and my fastqc data is as attached. is this correct?
01 April 2024 3,354 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...
05 August 2024 5,311 0 View