The use of bleach to deactivate guanidine hydrochloride containing buffers is well documented to be hazardous.
What is a suitable alternative disinfectant?
I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...
26 June 2024 4,522 0 View
Dear Sir/Madam, I am currently working on a beam shaping problem and have referenced the Metalens example provided on the Ansys Lumerical website. In that example, a forward propagating plane wave...
14 June 2024 4,333 0 View
My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...
10 June 2024 6,253 0 View
Does anyone have guidance on coating 96-well plates with collagen-I? Looking for a protocol. We are using Corning® Collagen I, Rat Tail, 100 mg.
22 April 2024 6,835 1 View
So I'm trying to determine what radical this is captured with DMPO. How do I determine and calculate what radical this is? Thanks!!
06 April 2024 7,084 3 View
As someone engaging in ethnographic research, are we expected to disclose transcribed data to the journal where we would like publish an article?
03 March 2024 3,653 3 View
Our lab is trying to use in vitro transcription to create mRNA of our inserted on a pcDNA 3.4 TOPO plasmid. I noticed it does not have a T7 promotor sequence. Are there other available promotors...
26 February 2024 1,852 1 View
More and more, teaching ETHICS has become an important part of the college curriculum, but teaching it has not always been easy or up to part with the generational changes in the student...
02 January 2024 7,862 6 View
I am trying to prepare some custom DNA binding solutions for purifying DNA with silica columns. I have prepared one with 4 M guanidine thiocyanate, 30% isopropanol, and 10 mM Phosphate buffer, pH...
03 December 2023 2,197 0 View
I am currently exploring on the concept of "Organizational Ambidexterity". I would love you give me an insight on the way to design its dimensions in a three level scale, where it will depict its...
27 October 2023 3,058 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...
05 August 2024 8,025 7 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View