Can we use a palindromic sequence having dsDNA labelled with fluorophores for fret experiments ?

06 October 2021 1 6K Report

can we use a palindromic sequence having dsDNA labelled with cyanine fluorophores for fret experiments ?

Some things to think about:

Will the FRET donor and acceptor be close enough together for significant energy transfer to occur?

Will the DNA sequence have a tendency for a single strand to form a hairpin, which would compete with the formation of double-stranded DNA?

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