Does anyone know the name of a plate (I hope it does exist) where each well is or can be divided into two chambers by means of a filter, which would allow a co-culture experiment WITHOUT the two bacterial or cell cultures mixing with each other? To be exact, allowing the broth to move freely, but restricting each type of bacteria to a specific chamber?
I have come across some transwell inserts, but not sure if that is what I am looking for.
If you know the name of the product, please share it.
Thank you!
Hi Leonid,
Please see the following link
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032528/
Hope this helps.
Sincerely,
Manisha
Hello Mr Ouchanov. You may try Transwell inserts. These inserts have different pore sizes, which you can choose based on the size of your target bacteria. For example, if your bacteria have been known to be between the size of 0.6-1.5 um per cell, then you can use the Transwell inserts of sizes 0.22 um or 0.45 um, because the cell culture mediums can still pass through, but the bacteria will not be able to. These inserts have different properties too - some are PET, polycarbonate, etc. If you want easier cell visibility, it will be best to go for PET since polycarbonate membrane inserts have very poor cell visibility.
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