High-Performance Liquid Chromatography

High-performance liquid chromatography (sometimes referred to as high-pressure liquid chromatography) is a chromatographic technique used to... | Contact experts in High-Performance Liquid Chromatography to get answers

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Questions related to High-Performance Liquid Chromatography

Suitable buffers for rehydrating lyophilized cultures?

I would like to request recommendations for suitable buffers for rehydrating lyophilized cultures. After rehydration, I need to store these cultures for two to three weeks at -200C while...

last month 1,225 2 View

How do researchers ensure that engineered viral vectors used in gene therapy are safe?

In my coursework, I am studying about viral vectors like adenoviruses and lentiviruses that are used for gene therapy. I understand that safety is a major concern, especially regarding insertional...

last month 9,108 1 View

Can preclinical and clinical investigations on botanicals such as papaya advance personalized medicine approaches in anemia management?

1. Anemia remains a multifactorial condition with varied etiologies and patient responses to therapy. Conventional treatments often overlook individual biochemical and genetic variability....

23 August 2025 5,527 0 View

Give us some drugs as examples 🙏?

How can nanotechnology-based drug delivery systems improve the therapeutic efficacy and reduce the side effects of conventional drugs?"

18 August 2025 3,087 2 View

How can I perform DPPH assay on Andrographolide?

I've been trying to do the DPPH assay on Andrographolide for weeks, but the result does not show any antioxidant effect on the compound. I use 0.1 mM of DPPH and the starting concentration is 1000...

15 August 2025 8,195 6 View

PhD Research Proposals in Business Administration, Management Sciences, Finance, or Islamic Finance to Strengthen My Application?

Request for Prepared PhD Research Proposals I am in the process of applying for a PhD in Business Administration, Management Sciences, Accounts, Finance, or Islamic Finance. To strengthen my...

10 August 2025 8,524 5 View

What does it mean by very high RMSD value for the protein structures superimposed through PyMol?

The superimposition of the wildtype and mutant structures using PyMol resulted in high RMSD values such as 22.694 ,23.491. But when checked through US-alignment the values were 3.31 and 2.41. why...

13 July 2025 2,824 2 View

How to avoid moisture from hygroscopic solid plant sample as long as possible means enhance self of formulation remain as solid state?

02 July 2025 8,100 0 View

How hydrophilic drug go through oral mucosa?

In the case of oral film, if hydrophilic drug that is not lipid soluble, is used as API, how does it cross the oral mucosal barrier to reach the bloodstream?

22 June 2025 7,809 3 View

How to increase the chances of homologous recombination for recombinant adenovirus production in prokaryotic cells ?

Hello everyone, I am trying to perform homologous recombination of the shuttle vector and pAd vector in BJ5183 cells. But it has been largely unsuccessful to date, and it is also difficult to...

11 June 2025 1,032 0 View

Why my ASNIII media is precipitating?

Hi i am trying to prepare ASNIII media but everytime when i autoclave the media it forms white precipitate is there anyway to avoid precipitation

08 June 2025 2,520 1 View

Which flavonoid enhanced bioavailability of anticancer drug?

Flavonoids anticancer Drug

07 June 2025 4,341 3 View

[Troubleshooting] After adding RIPA lysis buffer (HY-K1001) to cells, white floccules appear. Is this normal?

After adding RIPA lysis buffer (Cat.No.: HY-K1001, bought from MCE) to cells, white floccules appear. Is this normal? When I used RIPA lysis buffer to treat adherent/suspended cells, I observed...

22 May 2025 6,136 1 View

What is key difference between metabolic footprinting and metabolic fingerprinting?

Does metabolic footprinting focus on extracellular metabolites, and metabolic fingerprinting focuses on intracellular metabolites? I have a confusion between these two terms.what is key difference...

20 May 2025 5,662 1 View

What is the surface area of quinine?

I'm looking for any data regarding of quinine's surface area.

10 May 2025 4,009 0 View

Autodock vina : i am running autodock vina from command prompt. i get ligandout.pdbqt file generated. how to visualize in pymol?

i am running autodock vina from command prompt. i get ligandout.pdbqt file generated. autodock vina runs fine. but i am unable to visualize in pymol. even when i see in pymol, i could see only...

30 April 2025 7,401 3 View

Is this a comet (second try)?

Good evening This is my second time experimenting with the comet assay. Specifically, we are running PBMC samples and using silver stain for visualization. Could this images observed under the...

11 April 2025 9,354 2 View

How to isolate Jurkat cells from adherent co-culture without affecting CD69 expression?

After co-culturing adherent cancer cells and suspension cells (Jurkat), I want to harvest the Jurkat cells for flow cytometry (FACS). However, the two types of cells are strongly attached to each...

09 April 2025 5,543 2 View

What is the effectiveness of clay soils in filtering pollutants?

structure of clay molecules

04 April 2025 4,817 3 View

Can we use DAD detector for Sucrose analysis?

Sucrose Analysis using DAD detector and what type of column we can use C18 or amide column?

25 March 2025 8,269 0 View

I have used stearic acid as lubricant in Tolperisone HCl coated-tablet formulation. Can stearic acid affect on disintegrating time of coated-tablet?

For instance, the disintegrating time of core tablet is 5 minutes, but after coating, disintegration time increases to 10 minutes. This mean the disintegration time of tablet always increase to...

24 March 2025 2,739 2 View

Iam looking how to make standard for LCMS?

I'm new to LCMS technique and need some assistance. Could someone provide a reference or detailed protocol for preparing an isoleucine standard for LCMS analysis? I aim to quantify the isoleucine...

19 March 2025 3,422 4 View

CROs for Lentivirus production?

Does anyone have direct experience with these companies for providing lentivirus: VectorBuilder Genscript PacGene? If so, can you share your experiences (good/bad/in-between)? If there are...

18 March 2025 4,812 3 View

I have a 11kb fragment and I am trying to do IVT but every time I perform IVT a smear like band observe also it doesn't match the size any solution?

I am using 1% Agarose gel for running RNA band and the kit I am using is T7 NEB IVT kit I have also used RNase Inhibitor but nothing changed.

15 March 2025 3,239 2 View

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