High-Performance Liquid Chromatography

High-performance liquid chromatography (sometimes referred to as high-pressure liquid chromatography) is a chromatographic technique used to... | Contact experts in High-Performance Liquid Chromatography to get answers

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Questions related to High-Performance Liquid Chromatography

How can formulate Phosphorus 52 and Potassium 34 in liquid ?

formulate Phosphorus 52 and Potassium 34 in liquid

04 June 2024 7,540 2 View

Does freeze-dry damage crystals?

I want to apply freeze-drying to remove high-boiling point solvents while preserving the integrity of my crystals. I want to know whether there is any previous study about this method.

26 May 2024 7,237 1 View

How to get 192 of CeO2 atoms coordinates using Vesta?

Hi, Attached here is the CIF file of CeO2. One of the paper mentioned that they have used 192 CeO2 atoms constructed by 4*4*4 primitive cells. To generate the coordinates, I have opened it...

13 May 2024 7,892 5 View

What percentage of global crop production depends on reproductive seeds?

What percentage of global crop production depends on reproductive seeds?

10 May 2024 1,352 3 View

Interpretation of indirect effects with non-fitting components?

Hello all, I am running into a problem I have not encountered before with my mediation analyses. I am running a simple mediation X > M > Y in R. Generally, I concur that the total effect...

06 May 2024 7,407 4 View

This is a step in synthesis of morphine. Suggest how this is accomplished?

This is Problem K7 from Chapter "Alkaloids" (Special Topic K), pg 1004.

04 May 2024 8,780 0 View

What happens when food energy passes from herbivores to carnivores & energy enter an herbivore and travels through an omnivore to reach a carnivore?

What happens when food energy passes from herbivores to carnivores and how does energy enter an herbivore and travels through an omnivore to reach a carnivore?

25 April 2024 3,267 3 View

Can a potent form of Caffeine be made?

Is it possible to do a Structure Activity Relationship for Caffeine given that it's structure is similar to that of Adenine. Maybe analogues can be made using caffeine as lead compound....

23 April 2024 8,438 0 View

How to quantify Short Chain Fatty Acid in Stool samples of mice?

I am doing experiments in mice model of metabolic disorder. For that I have to do quantification od 3 major short chain fatty acid namely acetate propionate and butyrate. I had earlier tried...

16 April 2024 3,249 0 View

Why am I getting low isotopic enrichment in my expressed protein even though I am using labeled media?

I am expressing a membrane protein (about 15 kDa) in BL21(DE3) cells. The expression is good, and the yield is decent, but each time I checked the mass with a TOF mass spec, the mass I get is...

12 April 2024 7,126 3 View

Que peut apporter le sport au langage des enfants qui présentent des problèmes de langage?

l'acquisition du langage chez les enfants qui présentent des problèmes de langage par la pédagogie sportive

06 April 2024 3,659 2 View

Is it possible to dissolve or liquify lipids in pure water by heating?

Lipids contains hydrophobic and hydrophilic moieties, and it is generally insoluble in water. So my question is, whether is it possible to liquify or dissolve the lipids in water by attaining its...

20 March 2024 1,119 3 View

How display the SNPs positions along a chromosome on the top of my LD plot which provided by Haploview?

Hi, I have provided my LD plot by Haploview and I used Linkage Format (.ped & .info) to this aim. I am wondering how could I have the gene structure on the top of my LD plot ? I mean I need...

07 March 2024 5,114 2 View

Solvent evaporation at low temperature?

Hello, how can I evaporate Et2O or pentane at low temperatures (room temperature or ice in the rota vap) from volatile compounds the best way? And how long does it usually take to remove all...

02 March 2024 215 7 View

Why does band gap increase as particle size decreases and why does the Fermi level for an extrinsic semiconductor move with temperature?

Why does band gap increase as particle size decreases and why does the Fermi level for an extrinsic semiconductor move with temperature?

29 February 2024 2,163 0 View

Could an abundance of exposed positive charge on the surface of proteins affect size exclusion chromatography performance?

Hello, I am analyzing purified antibodies using a superdex 200 increase 10/300 GL column. Occasionally, I observe tailing at the peak, particularly with antibodies that have high surface...

26 February 2024 2,322 3 View

Why a hydrophilic protein is coming in pellet after overexpression?

A soluble protein is coming in pellet, which should come in supernatant. How to resolve this issue?

20 February 2024 9,756 1 View

What is the Gold Standard test to identify hypervirulent Klebsiella pneumoniae?

I am Searching for the gold standard test to identify hypervirulent Klebsiella pneumoniae and differentiate it from classical Klebsiella pneumoniae.

20 February 2024 2,243 1 View

Gaussian Connectivity Error?

Hi! I am working on a thesis project using Gaussian to model reactivtiy in compounds and I keep getting the error message " End of file reading connectivity." I have looked through Gaussian...

04 February 2024 5,833 2 View

Is the concentration of cell protein related to cell size or number?

When calculating the content of certain substances, it is common to use protein concentration as a standard. My question is this protein concentration, what is the standard indicator? Is this cell...

31 January 2024 5,013 0 View

How to find out Laccase enzyme's activity other than using ABTS?

After purifying Laccase enzyme (Bacterial origin), I tried performing enzyme assay using ABTS which is a known substrate. Still I am unable to validate its activity using the said substrate....

22 January 2024 5,116 0 View

Querry on maintainance of bacterial cultures?

I am working in the field of Medical Microbiology, My sample size is 300. The biggest problem with such a high number of microbial cultures is contamination and difficulty in maintaining them for...

20 January 2024 1,576 5 View

Which is prefferable to use for publications and why? My personal email (g-mail/yahoo etc) or official (institution) email?

Which is prefferable to use for publications and why? My personal email (g-mail/yahoo e.t.c) or official (institution) email. Especially in cases where I have a choice? Does any of the 2 have...

13 January 2024 4,561 3 View

Do you know the largest and reachest Ag mine in world?where it is?

I want to know more about the Ag deposits.

12 January 2024 3,708 1 View

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