Chromatography

Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary... | Contact experts in Chromatography to get answers

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Questions related to Chromatography

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

20 February 2021 5,781 2 View

Why is anionic exchange chromatography more common than cationic exchange chromatography?

Is it true that there are more negative charged proteins?

19 January 2021 8,416 2 View

Is it possible to separate Supleco 37 Fame mix using HP-5MS column?

Hi everyone, I am new to FAME analysis. I am trying to separate Supleco 37 Fame mix using the HP-5MS (30 m * 0.25 mm ID * 0.2 um thickness) column using the following protocol: System: Agilent...

20 December 2020 7,544 4 View

Can I split Chromas double peaks?

I have a chromatography from a DNA sequence which is a heterozygote for a 3 pb deletion. This makes that for now on all the sequence have double peaks. An idea I have is to split it, so I have...

28 November 2020 1,570 3 View

How to change normal H silica gel to C silica gel for reverse chromatography.??

29 September 2020 9,770 5 View

Is there any cost-effective, simple and efficient way to analyse and estimate the amount of vitamins in algal samples?

I am currently pursuing my research in Phycology. I'd like to know if there's any simple and cost-effective method to detect and analyze nutrients and vitamins in the algal matter. The experiment...

19 September 2020 4,265 3 View

Size-exclusion chromatography for the isolation of EVs?

Dear all, I am currently doing research on extracellular vesicles (EVs). I'd like to improve the isolation method by trying out size-exclusion chromatography (SEC) compared to serial...

08 September 2020 4,569 5 View

How to separate mixture of two organic compounds using size exclusion chromatography?

My organic compounds have a molecular weight in the range of 2500g/mol and other is 1066.22 g/mol. aThe TLC results show three spots. But the main problem with my compounds are acid sensitivity of...

21 August 2020 6,284 3 View

What is the best chromatography (type, resin..) to separate blood lipoproteins among each other?

Dear all, I am trying to understand which is the "golden standard" method to separate lipoproteins from the blood (LDL, HDL, IDL, chylomicrons...). I found some methods online, and apparently...

11 August 2020 4,872 5 View

Methods to attach glutathione agarose resin to glass slide?

Hello, I am looking to capture a GST tagged protein on a glass slide. I was wondering if anyone had any experiences with glutathoine agarose resin. I realize this is commonly used in affinity...

14 July 2020 1,960 3 View

Need help in gas chromatography analysis report?

Hi Everyone!! I am attaching the gas chromatography analysis report here. The shape of peaks in my calibration report doesn't look fine.is'nt it? What in your opinion is the reason 1) of these...

21 June 2020 1,599 3 View

I need a protocol for Cas9 expression in E coli?

Hello Dear researchers, I am trying to express Cas9 vector in E coli but finding difficulties. After IPTG induction I am unable to get any protein(Cas9) via His-tag chromatography. Also, there is...

18 June 2020 1,306 2 View

What features are used for principal component analysis(PCA) of chromatography?

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19 May 2020 1,795 2 View

Ion exchange vs Size exclusion chromatography based on yield and purify?

What is differences between ion exchange and size exclusion chromatography based on yield and purify. Which one has higher purity/yield and reliable?

19 April 2020 4,018 5 View

Waters Empower 3- Recover Deleted Sample Set?

Right before my lab shut down, I ran several samples using Empower 3 and started analyzing the data remotely from home afterwards. Without realizing what I was doing, I deleted the sample set of...

15 April 2020 4,366 3 View

Is there resolution in size exclusion chromatography?

I want to isolate a 25kDa-protein, but impure proteins are different in size within 10kDa. how can we tell whether the SEC column could separate proteins of interest from impure ones? I attach...

12 April 2020 8,003 6 View

A question regarding Response factor in GC. what does area count mean?

i was reading about response factors in GC and i encountered the term area count, I was wondering if anyone can explain it to me, i have a general idea about the meaning but i'm not sure. does...

31 March 2020 8,020 1 View

How to separate benzene and cyclohexane on a GC?

Hi! I'm currently working on a system where benzene and cyclohexane are two of the main analytes. (Alongside phenol, cyclohexanol and hexadecane.) I have attempted to quantify my products by...

27 February 2020 9,607 10 View

Organofluorine compounds in Kombucha?

Kombucha, a fermented Tea with low pH, contains substantial Fluoride in the form of Hydrogen Fluoride as well as the ion. Kombucha has been associated with many adverse effects, including acute...

13 February 2020 549 1 View

Sep-pak C18 cartridge alternatives?

I am currently conducting a study about the formation of free EPA in raw oysters treated with acidic sauces. For separating the polar and nonpolar lipids, I will be using Sep-pak C18 catridges....

09 February 2020 8,424 3 View

What is a suitable purification method for big dextrans (> 2000 kDa) beside size exclusion chromatography?

Is capillary electrophresis or ion exchange chromatography a convenient method when charges are introduced into the dextran?

07 February 2020 8,078 6 View

Limit of sample loading in Biotage flash purification system cartridges?

I am using Biotage Isolera one. There are different columns available based on amount of stationary phase. Does anyone know/have a chart on maximum limit of crude sample that can be loaded into...

06 February 2020 9,287 6 View

Side reactions of Vitamin D derivatization with PTAD?

Dear all, I am doing Vitamin D derivatization with PTAD like described in this paper Derivatization methods for LC-MS analysis of endogenous compounds (reaction mechanism is in Figure 3) to...

23 January 2020 3,910 3 View

How do i dilute the sample before GC X GC analysis? How do I know the amount of the sample to add to the solvent? Do I have to order standards?

I want to start my project. It involves the use of two-dimensional chromatography. I want to use sample dilution prior to analysis and my solvent is DCM. How do I go about it? How do I know the...

21 January 2020 641 6 View

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