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Questions related from Nurul Adhwa Rahman
I am working on a co-culture of two different cells using a polycarbonate membrane Transwell inserts to see if either one or both cells grow better together versus monoculture of the cells by...
03 July 2016 7,787 6 View
I have provided picture of the fungal growth on sabouraud agar, and its reverse morphology, also the staining using lactophenol cotton blue (LPCB).
18 November 2015 3,671 25 View
16 November 2015 2,873 2 View
I have Mueller-Hinton broth (CM0405, OXOID). However as stated in all CLSI documents, I should prepared it to be "cation-adjusted Mueller Hinton broth (CAMHB)" for fastidious bacteria. In the...
09 November 2015 4,294 3 View
I am no expert in fungi. However I need to identify few fungal growth on sabouraud agars. Are there any easy to read and recommended book that has both the culture growth images and the...
08 November 2015 2,523 9 View
I am interested in the time-kill assay. However which is the original source of the methodology? I have read that CLSI has produced a document on the approved guideline titled, 'Methods for...
13 October 2015 3,572 6 View
My DMEM has 3.7g/L of Sodium bicarbonate, instead of 1.5g/L (I only have this DMEM), suggesting that my sensitive endothelial cells need 10% CO2 incubator. However I am working in an academic...
13 September 2015 9,116 5 View
I was reviving endothelial cells. When I added the cells to the DMEM, the medium immediately changes colour to purple. It was like a magic! Then one day after that, the medium returns back to red...
08 September 2015 3,824 10 View
Hello. My team has just revived bEnd.3 yesterday from a cryovial stored in liq nitrogen, purchased from ATCC, following the protocol stated by ATCC. After 24 hours, we only saw about 1-2 spindle...
13 August 2015 6,789 3 View
I am interested to understand the rationale behind the method of use (as mentioned in the file attached) as follows: 1. Why we cannot allow the suspension to sit at room temperature for longer...
29 June 2015 10,137 3 View
Immunofluorescence staining is applied to cells either as in-vitro cell culture or in vivo animal models, and localisation of infectious agents is determined via the immunofluorescence...
06 June 2015 9,126 4 View
Has anyone ever made their own PTFE-coated microscope slides? The ready-made PTFE-coated microscope slides are sold at a high price in my country, so I am wondering if it is more cost-effective if...
13 May 2015 1,169 1 View
Other than in brain heart infusion (BHI), in which other medium can Neisseria gonorrhoeae grow? I am afraid that I do not have BHI right now.
13 May 2015 1,286 6 View
I was going to get Kit Fast-Read 102 from Biosigma, but then the supplier in my country is selling it for BND$8K per box of 100 slides. It is very expensive for such a disposable material. May I...
27 March 2015 4,494 1 View
The powdered crude plant extract could be stored at 4'C for how long? Could this be for 5-10 years? Are they any special precautions that I should be aware of in storing the extract?
20 March 2015 8,338 6 View
Hello everyone. Where do you think I could easily get Pseudomonas aeruginosa from the environment? I used to easily culture Vibrio parahaemolyticus from different shellfish on Vibrio chromogenic...
19 March 2015 5,200 16 View
Have anyone tried different brands of non-fat dry milk for western blot, as blocking solution and antibody diluent? Would all brands work fine? I only know one Japanese brand that is widely used...
24 February 2015 7,644 4 View
I've got a good and new set of CLED agars (not expired). I grew Proteus mirabilis on it, and interestingly, it still swarms on the agar, although slower if compared to its growth on a nutrient...
13 February 2015 4,571 3 View
I have a lot of Bacillus cereus cultured on nutrient agars, but kept inside the 4°C freezer after 24 hours in 37°C incubator. Then I used the following protocol to do endospore staining: 1....
04 February 2015 4,187 11 View
An undergraduate has a contaminant growing on her 48-hour nutrient agar. She was supposed to be growing E.coli, but another organism grew and swarmed over her agar plate. I am genuinely interested...
02 February 2015 8,022 6 View
"Alexa Fluor® 488 dye conjugate showed a 15–30% better signal-to-noise ratio than the DyLight™ 488 conjugate" - How true is this? Does anyone have any experience that says the opposite? Do Alexa...
22 December 2014 1,841 6 View
The official BD website only states the function and what it is for. They say that it is a chromogenic/fluorogenic medium used to detect and enumerate Escherichia coli and total coliforms in...
18 December 2014 8,553 3 View
If I were to use a clinical isolate of Streptococcus pneumoniae, without any information on the serotype, which antibody should I apply to view S. pneumoniae under the fluorescence microscopy? I...
15 December 2014 2,137 1 View
Trypan blue is known to be carcinogenic. So, I am very careful in purchasing this product. From your experience, which one is best to handle the trypan blue - in powder form (to dilute) or in the...
05 November 2014 927 3 View
