For various reasons, I need to avoid DMSO with my cells. So I wonder if isopropyl alcohol (2-propanol) could be used instead of DMSO to create my stock solutions library?
I have learned that the polarity index of isopropyl alcohol is 3.9 (H20= 10.2; DMSO = 7.2). So, theoretically, it could be a better solvent for non-polar compounds than DMSO. Moreover, it is physiological (the normal concentration in human plasma is 80 uM, against 0 for DMSO). And, the cherry on the cake: it is sterilizing (no need for sterile filtration of the solution, so no loss of solution during filtration) and it will remain liquid at -20 (no freezing-thawing). Furthermore, once added to the saline solution, it will be salted out and then evaporate, so only traces will eventually be present in the culture medium.
Thus, the theoretical part of the problem seems to be very advantageous. But according to the literature, it is only rarely used. What is reason? I haven't seen any booby trap?
thank you
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