Hello to all
I observed a strange phenomenon with CHO-K1 following the change of serum batch (it is surely the toxicity of this batch but I am very curious about the possible nature of this phenomenon. In practice, I will simply change serum. But the question remains open)
At the beginning of the culture, CHO-K1 grow normally. I observe a normal triangular morphology (round to cubic). The growth is fast as usual. But, once at confluence and not before, they start to take the shape of very elongated cells in parallel arrays evolving like fibroblasts.
I tried to dilute them as much as possible at the beginning of the culture. No change. They take a normal amount of time to grow to confluence, then they become fibroblasts-like.
Has anyone seen this kind of phenomenon? It is probably toxicity in the serum, but usually the toxicity is greater if you dilute the cells (" dilution death "). This is not the case this time. Any ideas would be helpful.
Thank you.
Hi Lëonid,
Once happened to me and searching in literature I found some articles relating cAMP with this morphological change (PMIDs 2993305, 232579, 17557277, 20840080).
Hi Maria
thank you very much for your quick reply
the information you have shared sounds very interesting. Thank you, I will learn a lot thanks to you.
thanks again to Maria
effectively, this phenomenon (reverse transformation of chinese hamster ovary cells) is well known and linked to the intracellular level of cAMP.
some other related articles :
cAMP Induced Alterations of Chinese Hamster Ovary Cells Monitored by Mass Spectrometry
Morphological reverse transformation of Chinese hamster ovary CHO cells and surface fibronectin
Reverse transformation of Chinese hamster ovary cells by methyl xanthines. Structure-function relationships
Independent induction of morphological transformation of CHO cells by receptor-activated cyclic AMP synthesis or by receptor-operated calcium influx
Shapes of cells spreading on fibronectin- measurement of the stellation of BHK21 cells induced by raising cyclic AMP, and of its reversal by serum and lysophosphatidic acid
- Badges
- Science topic
- Similar topics
- Computer Science
- Program