Hello,
I have a somewhat weird question:
has anyone had problems with campylobacter growing in the second half of the strip GLU through ERO?
Have you managed to correct it?
There is some kind of conflict between the image (where you are aupposed to see blurred lines due to bacterial growth, available online) and instructions (where you need to transfer 80-100 ul of the inoculate into the cupule). However, 80-100 ul is not neraly enough to cover those lines and the gel is not liquid enough to transfer it over the lines by declining the strip a little.
Dilemma
Hi Leonid, I'll be honest, I haven't used the API Campy kit but looking online at the instructions, the protocol seems very similar to other API kits that I've used for Lactic Acid Bacteria.
chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.mediray.co.nz/media/15786/om_biomerieux_test-kits_ot-20800_package_insert-20800.pdf
For the URE to PAL tests, you need to add 80-100ul of the bacterial suspension. However, for the GLU to ERO tests, you need 150ul of the bacterial suspension API AUX Medium (which is a separate 5ml vial that should be provided). Mix well and then add this suspension to the sugar tests.
Hopefully that helps.
Hi Leonard. Thank you. Yes, the instructions say to transfer 150 ul from the saline mixture to AUX, mix and then fill the capules, but it fails to tell you the volume. Basically, you need to fill them with over 200 uL of the mix.
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