If anyone has any idea about the expression of cPLA-2 in A549 cells, please let me know. What are the time points to check its expression in cells?
If you want to treat cancer cells (i.e. A549 cells) with some stimulus (i.e. LPS) and want to check the effect of any flavonoid, what is the best way for starvation and treatment? Should I...
02 March 2017 7,109 5 View
Do anyone have any information about expression level of 5-lipooxygenase in airway epithelial cells, especially in A549 cells!
01 February 2017 9,941 2 View
As it is not easy to get bands for MMP-2 and MMP-9 in western blotting, if anyone have some idea regarding this please let me know.
31 December 2016 5,762 4 View
I want to design some experiments on normal but transformed airway epithelial cells. I know about BEAS-2b but it requires collagen coating. Please suggest some more cell lines.
31 December 2016 2,291 2 View
Morphology of cells is fine. No change in media color, pH or proliferation rate. we are observing black debries+ some thread like structure. They are not affecting cell's morphology. What could be...
09 October 2016 4,017 8 View
Media is fine. other things are also OK. Derbies are not present within the cells. They are not affecting cell's morphology or anything and mainly present in background. but its in large amount...
08 September 2016 8,364 4 View
Can anyone please tell me proper time period for serum starvation in A549 cells?
07 August 2016 7,437 3 View
I want to check expression of IL-1 B (active form of IL-1B) from media. can anyone tell me which protocol ( specifically protein load after lyophilization) will be helpful?
06 July 2016 2,001 4 View
I want to use MMP-2 / MMP-9 common inhibitor for my studies. So many inhibitors are there in market.Kindly suggest some good inhibitor.
04 May 2016 1,677 6 View
I am working on airway epithelial cells. i want transformed normal airway epithelial cells. Please guide me.
01 February 2016 9,684 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View