As it is not easy to get bands for MMP-2 and MMP-9 in western blotting, if anyone have some idea regarding this please let me know.
Dear Hina. It sounds like you have spent a lot of time testing antibodies against these targets. Finding suitable antibodies is a huge problems for scientists all over the world, and it is therefore crucial we share the information we obtained by using these antibodies.
www.pAbmAbs.com is a website where scientists can share the experience regarding commercially available antibodies that they obtained through their research. I can see that nobody has shared information about antibodies against mmp-9 and mmp2, and i therefore hope you will share your experience? This would help others scientists that are also looking for suitable antibodies against these targets.
Kind regards,
Simon Molgaard
According to Biocompare there are currently at least 828 MMP2 Western Blot Antibodies across 42 suppliers and 912 MMP9 Western Blot Antibodies across 41 suppliers. We could narrow this down a bit if you need the antibodies for one species only. Needless to say that scientists find themselves overwhelmed when they need to shop around for the one antibody they need. Aeonian Biotech is starting up this year by offering assistance finding the right antibody in the market so you run no risks of wasting funds and time on the wrong and badly performing products.
Hi Hina,
For MMP-9, you can use the anti-goat MMP-9 from R&D systems (dilution 1/2000).
You can look at my publication:
https://www.ncbi.nlm.nih.gov/pubmed/27301579
S.
Hello,
I would recommend a free antibody database available at labome.com. For MMP-2 antibodies you can check the following link: https://www.labome.com/review/gene/human/MMP-2-antibody.html. Abcam has MMP-2 antibody (Abcam, ab92536) used in western blot (knockout) on mouse samples (ref: Hughes et al., Am J Physiol Heart Circ Physiol. 2014). Also, Santa Cruz Biotechnology provides MMP-2 antibody (Santa Cruz, sc-13595) used in western blot on human samples at 1:500 and on mouse samples at 1:500. In addition, Invitrogen has MMP-2 antibody (Lab Vision, MS-567-P0) used in western blot on mouse samples (fig 3a) (ref: Seo et al, Brain Res 2009). GeneTex offers MMP-2 antibody (GeneTex, GTX104577) used in western blot on human samples (Gao et al., Sci Rep 2016). EMD Millipore has MMP-2 antibody (MD Millipore, MAB3308) used in western blot on human samples (fig 3) (ref: Yang et al, Sci Rep 2016).
For MMP-9 antibodies you can check the following link: https://www.labome.com/review/gene/human/MMP-9-antibody.html. Abcam has MMP-9 antibody (Abcam, ab38898) used in western blot on mouse samples (ref: Pang et al, Oncotarget 2016). Santa Cruz Biotechnology offers MMP-9 antibody (Santa Cruz, Sc-21733) used in western blot on human samples at 1:200 (ref: Eterno et al, Sci Rep 2016). Invitrogen offers MMP-9 antibody (Thermo Scientific, MS-817-P0) in western blot on human samples at 1:200 (ref: Dayer et al, J Biol Chem 2015). GeneTex also has MMP-9 antibody (GeneTex, GTX100458) used in western blot on human samples at 1:1000 (fig 2) (ref: Gao et al, Sci Rep 2016). EMD Millipore offers MMP-9 antibody (Millipore, MAB13415) used in western blot on human samples at 1:200 (fig 2) (ref: Wei et al, Sci Rep 2016). You can see the description of these antibodies and references following the provided links.
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