What is the best way for starvation of cancer cells?

02 March 2017 6,967 5 View

Can anyone tell me which company gives best antibodies for MMP2 and MMP-9 proteins?

As it is not easy to get bands for MMP-2 and MMP-9 in western blotting, if anyone have some idea regarding this please let me know.

31 December 2016 5,629 4 View

Can anyone tell me about the expression of cPLA-2 in A549 cells?

If anyone has any idea about the expression of cPLA-2 in A549 cells, please let me know. What are the time points to check its expression in cells?

31 December 2016 2,716 0 View

Which normal / immortalized airway epithelial cells are best for study?

I want to design some experiments on normal but transformed airway epithelial cells. I know about BEAS-2b but it requires collagen coating. Please suggest some more cell lines.

31 December 2016 2,144 2 View

We are observing black thread like particles in cells. what could be the reason?

Morphology of cells is fine. No change in media color, pH or proliferation rate. we are observing black debries+ some thread like structure. They are not affecting cell's morphology. What could be...

09 October 2016 3,883 8 View

We are getting black particles/ derbies in our cells. What could be the reason?

Media is fine. other things are also OK. Derbies are not present within the cells. They are not affecting cell's morphology or anything and mainly present in background. but its in large amount...

08 September 2016 8,215 4 View

What is the appropriate time period for serum starvation in A549 cells?

07 August 2016 7,302 3 View

Can anyone suggest me how to do western blotting for IL-1 beta from media?

I want to check expression of IL-1 B (active form of IL-1B) from media. can anyone tell me which protocol ( specifically protein load after lyophilization) will be helpful?

06 July 2016 1,863 4 View

Can anyone suggest me potent MMP-2/MMP-9 common inhibitor?

I want to use MMP-2 / MMP-9 common inhibitor for my studies. So many inhibitors are there in market.Kindly suggest some good inhibitor.

04 May 2016 1,534 6 View

Can anyone suggest, where to obtain transformed airway epithelial cells ?

I am working on airway epithelial cells. i want transformed normal airway epithelial cells. Please guide me.

01 February 2016 9,566 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View