Hina Agraval

14 Questions 28 Answers 0 Followers

Questions related from Hina Agraval

What is the best way for starvation of cancer cells?

If you want to treat cancer cells (i.e. A549 cells) with some stimulus (i.e. LPS) and want to check the effect of any flavonoid, what is the best way for starvation and treatment? Should I...

03 March 2017 7,065 5 View

What is the expression levels of 5-lipooxygenase in A549 cells?

Do anyone have any information about expression level of 5-lipooxygenase in airway epithelial cells, especially in A549 cells!

02 February 2017 9,898 2 View

Which normal / immortalized airway epithelial cells are best for study?

I want to design some experiments on normal but transformed airway epithelial cells. I know about BEAS-2b but it requires collagen coating. Please suggest some more cell lines.

01 January 2017 2,244 2 View

Can anyone tell me about the expression of cPLA-2 in A549 cells?

If anyone has any idea about the expression of cPLA-2 in A549 cells, please let me know. What are the time points to check its expression in cells?

01 January 2017 2,805 0 View

Can anyone tell me which company gives best antibodies for MMP2 and MMP-9 proteins?

As it is not easy to get bands for MMP-2 and MMP-9 in western blotting, if anyone have some idea regarding this please let me know.

01 January 2017 5,725 4 View

We are observing black thread like particles in cells. what could be the reason?

Morphology of cells is fine. No change in media color, pH or proliferation rate. we are observing black debries+ some thread like structure. They are not affecting cell's morphology. What could be...

10 October 2016 3,969 8 View

We are getting black particles/ derbies in our cells. What could be the reason?

Media is fine. other things are also OK. Derbies are not present within the cells. They are not affecting cell's morphology or anything and mainly present in background. but its in large amount...

09 September 2016 8,312 4 View

What is the appropriate time period for serum starvation in A549 cells?

Can anyone please tell me proper time period for serum starvation in A549 cells?

08 August 2016 7,392 3 View

Can anyone suggest me how to do western blotting for IL-1 beta from media?

I want to check expression of IL-1 B (active form of IL-1B) from media. can anyone tell me which protocol ( specifically protein load after lyophilization) will be helpful?

07 July 2016 1,964 4 View

Can anyone suggest me potent MMP-2/MMP-9 common inhibitor?

I want to use MMP-2 / MMP-9 common inhibitor for my studies. So many inhibitors are there in market.Kindly suggest some good inhibitor.

05 May 2016 1,630 6 View

Can anyone suggest, where to obtain transformed airway epithelial cells ?

I am working on airway epithelial cells. i want transformed normal airway epithelial cells. Please guide me.

02 February 2016 9,648 1 View

Black particles in RPMI 1640 media. What could be the reason?

I have made RPMI 1640 media before 3 days. In tester plate, there are black particles. These particles are big and not like any other contaminant or debries.In higher magnification , they look...

02 February 2016 9,115 3 View

I use A549 cells for my study. Recently they are elongated . What could be the reason?

There is no contamination in cells. passage number is :30. Still after every passage they look more elongated. I use RPMI 1640 medium with 10% FBS.

01 January 2016 2,890 12 View

Can anyone help me with NAC solution preparation?

I want to check the effect of NAC on my cells. what's the method to prepare NAC in PBS? Can i prepare it in media?

07 July 2015 9,650 5 View