14 Questions 28 Answers 0 Followers
Questions related from Hina Agraval
If you want to treat cancer cells (i.e. A549 cells) with some stimulus (i.e. LPS) and want to check the effect of any flavonoid, what is the best way for starvation and treatment? Should I...
03 March 2017 7,065 5 View
Do anyone have any information about expression level of 5-lipooxygenase in airway epithelial cells, especially in A549 cells!
02 February 2017 9,899 2 View
I want to design some experiments on normal but transformed airway epithelial cells. I know about BEAS-2b but it requires collagen coating. Please suggest some more cell lines.
01 January 2017 2,245 2 View
If anyone has any idea about the expression of cPLA-2 in A549 cells, please let me know. What are the time points to check its expression in cells?
01 January 2017 2,806 0 View
As it is not easy to get bands for MMP-2 and MMP-9 in western blotting, if anyone have some idea regarding this please let me know.
01 January 2017 5,726 4 View
Morphology of cells is fine. No change in media color, pH or proliferation rate. we are observing black debries+ some thread like structure. They are not affecting cell's morphology. What could be...
10 October 2016 3,969 8 View
Media is fine. other things are also OK. Derbies are not present within the cells. They are not affecting cell's morphology or anything and mainly present in background. but its in large amount...
09 September 2016 8,313 4 View
Can anyone please tell me proper time period for serum starvation in A549 cells?
08 August 2016 7,393 3 View
I want to check expression of IL-1 B (active form of IL-1B) from media. can anyone tell me which protocol ( specifically protein load after lyophilization) will be helpful?
07 July 2016 1,966 4 View
I want to use MMP-2 / MMP-9 common inhibitor for my studies. So many inhibitors are there in market.Kindly suggest some good inhibitor.
05 May 2016 1,631 6 View
I am working on airway epithelial cells. i want transformed normal airway epithelial cells. Please guide me.
02 February 2016 9,649 1 View
I have made RPMI 1640 media before 3 days. In tester plate, there are black particles. These particles are big and not like any other contaminant or debries.In higher magnification , they look...
02 February 2016 9,115 3 View
There is no contamination in cells. passage number is :30. Still after every passage they look more elongated. I use RPMI 1640 medium with 10% FBS.
01 January 2016 2,891 12 View
I want to check the effect of NAC on my cells. what's the method to prepare NAC in PBS? Can i prepare it in media?
07 July 2015 9,653 5 View