I want to use MMP-2 / MMP-9 common inhibitor for my studies. So many inhibitors are there in market.Kindly suggest some good inhibitor.
Dear Hina, In what system do you want to inhibit the MMP2/9 activities? (For instance a simple approach is to use a metal chelating agent like o-phenanthroline, but it depends what you want to achieve).
Good luck! Simon
Dear Hina, In what system do you want to inhibit the MMP2/9 activities? (For instance a simple approach is to use a metal chelating agent like o-phenanthroline, but it depends what you want to achieve).
Good luck! Simon
Dear Hina,
I totally agree with Simon. If you are able to provide more information about your goals then it will be much more easier to suggest a common inhibitor of MMP2/9 that suits your purpose.
Metal chelating agent like o-phenanthroline or calcium chelating agent EGTA/EDTA will certainly will serve the purpose. But they lack specificities which may complecate your experimental observations.
Compound 5a is also available from Abcam. (http://www.abcam.com/mmp-2mmp-9-inhibitor-i-ab145190.html) or from Santa Cruz Biotechnology (http://www.scbt.com/datasheet-311429.html). Millipore also has this compound (http://www.emdmillipore.com/US/en/product/MMP-2MMP-9-Inhibitor-I---CAS-193807-58-8---Calbiochem,EMD_BIO-444241?bd=1).
Attached is an article you can find more info about compound 5a.
Hope that helps!
I suppose the first question is - do you want to inhibit activity in a 'cell-free' sample (eg conditioned culture medium, a biological fluid, or a tissue extract / homogenate) that contains MMPs? in which case the use of a chelating agent will work. If you wish to inhibit MMP activity in growing cells, then using a chelator will have a significant effect on the wellbeing of those cells!
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Proteins