What is the best way for starvation of cancer cells?

02 March 2017 6,967 5 View

What is the expression levels of 5-lipooxygenase in A549 cells?

01 February 2017 9,797 2 View

Can anyone tell me which company gives best antibodies for MMP2 and MMP-9 proteins?

As it is not easy to get bands for MMP-2 and MMP-9 in western blotting, if anyone have some idea regarding this please let me know.

31 December 2016 5,629 4 View

Can anyone tell me about the expression of cPLA-2 in A549 cells?

If anyone has any idea about the expression of cPLA-2 in A549 cells, please let me know. What are the time points to check its expression in cells?

31 December 2016 2,716 0 View

Which normal / immortalized airway epithelial cells are best for study?

I want to design some experiments on normal but transformed airway epithelial cells. I know about BEAS-2b but it requires collagen coating. Please suggest some more cell lines.

31 December 2016 2,144 2 View

We are observing black thread like particles in cells. what could be the reason?

Morphology of cells is fine. No change in media color, pH or proliferation rate. we are observing black debries+ some thread like structure. They are not affecting cell's morphology. What could be...

09 October 2016 3,883 8 View

We are getting black particles/ derbies in our cells. What could be the reason?

Media is fine. other things are also OK. Derbies are not present within the cells. They are not affecting cell's morphology or anything and mainly present in background. but its in large amount...

08 September 2016 8,215 4 View

What is the appropriate time period for serum starvation in A549 cells?

07 August 2016 7,302 3 View

Can anyone suggest me how to do western blotting for IL-1 beta from media?

I want to check expression of IL-1 B (active form of IL-1B) from media. can anyone tell me which protocol ( specifically protein load after lyophilization) will be helpful?

06 July 2016 1,863 4 View

Can anyone suggest me potent MMP-2/MMP-9 common inhibitor?

I want to use MMP-2 / MMP-9 common inhibitor for my studies. So many inhibitors are there in market.Kindly suggest some good inhibitor.

04 May 2016 1,534 6 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View