How can we define tumor micro environment?

I am interested to know the exact definition of tumor micro-environment in reference to FFPEs (Formalin Fixed Paraffin Embedded tissues) and in vivo both. Does it depend upon the physical distance...

09 October 2017 3,355 6 View

Serum starvation for synchronization of cells?

We synchronize cells for different experiments, but my question is in reference to drug treatment. If we synchronize cells by minimal or no media, should we add the treatment or drug in complete...

06 July 2017 1,605 5 View

How to calculate the molarity of a solution if we accidentally mix two aliquots of solution with different molarity ?

02 March 2017 5,610 3 View

Any idea of a good CD28 antibody?

I need a CD28 antibody preferably Mouse/Rabbit ANTI HUMAN. I want to use it with IHC or ICC. But kindly suggest the one working with Myeloma Cells. Any help would be appreciated.

11 December 2016 4,753 6 View

Can we store cells at 4 Degree aftre fixation with 4% PFA? and can they be usd further for cytospin, IHC staining or IF?

Hi All, I wanted to know if suspension cells can be stored at 4 degrees after fixation with 4% PFA for 20 minutes. After good washing if I store them for around a week at 4 in PBS, would it have...

02 March 2016 6,427 5 View

Is there any way to select Lentiviral transduced cells other than FACS sorting?

11 December 2015 9,302 1 View

Invasion Assay not working for myeloma cell lines. Could it be the pore size or chemo attractant?

I am trying Matrigel invasion assay for myeloma cell lines. I am using 8 microns membrane and coating the matrigel at 1: 3 conc. But am not getting results. I am using U266 cell line which is...

09 October 2014 3,711 4 View

What is the best method for characterization of HEK 293T Cells?

I have frozen vials of HEK 293T cells. Before using it in my experiments, I want to characterize it. Normally we can go for STR analysis but I want to do something less expensive yet standard such...

08 September 2014 7,669 4 View

Can anyone give me some information on scoring methods for IHC slides in myeloma?

I am staining myeloma samples for the expression of a panel of markers. When it comes to evaluate these slides, I cannot find any exact criteria defined in papers. Can anyone please help me with this?

04 May 2014 200 5 View

Can anyone help with the expression of androgen receptor in triple negative breast carcinoma cell line?

I have transfected androgen receptor in a triple negative breast cancer cell line. Normally, the androgen is expressed in cytoplasm and after treatment with DHT it translocates to nucleus. But...

04 May 2014 4,524 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

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28 February 2021 9,936 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View