How can we define tumor micro environment?

I am interested to know the exact definition of tumor micro-environment in reference to FFPEs (Formalin Fixed Paraffin Embedded tissues) and in vivo both. Does it depend upon the physical distance...

09 October 2017 3,355 6 View

Serum starvation for synchronization of cells?

We synchronize cells for different experiments, but my question is in reference to drug treatment. If we synchronize cells by minimal or no media, should we add the treatment or drug in complete...

06 July 2017 1,605 5 View

How to calculate the molarity of a solution if we accidentally mix two aliquots of solution with different molarity ?

02 March 2017 5,610 3 View

Can any one suggest the recipe of Acidified Ethanol and what percent of it is acceptable in tissue culture?

I need to dilute a drug Melphalan in acidified ethanol. The insert says3 drops of HCL in ethanol. Is it really fine to add without measuring 3 drops. Moreover I have found that Ethanol is...

02 March 2017 2,852 0 View

Can we store cells at 4 Degree aftre fixation with 4% PFA? and can they be usd further for cytospin, IHC staining or IF?

Hi All, I wanted to know if suspension cells can be stored at 4 degrees after fixation with 4% PFA for 20 minutes. After good washing if I store them for around a week at 4 in PBS, would it have...

02 March 2016 6,427 5 View

Is there any way to select Lentiviral transduced cells other than FACS sorting?

11 December 2015 9,302 1 View

Invasion Assay not working for myeloma cell lines. Could it be the pore size or chemo attractant?

I am trying Matrigel invasion assay for myeloma cell lines. I am using 8 microns membrane and coating the matrigel at 1: 3 conc. But am not getting results. I am using U266 cell line which is...

09 October 2014 3,711 4 View

What is the best method for characterization of HEK 293T Cells?

I have frozen vials of HEK 293T cells. Before using it in my experiments, I want to characterize it. Normally we can go for STR analysis but I want to do something less expensive yet standard such...

08 September 2014 7,669 4 View

Can anyone give me some information on scoring methods for IHC slides in myeloma?

I am staining myeloma samples for the expression of a panel of markers. When it comes to evaluate these slides, I cannot find any exact criteria defined in papers. Can anyone please help me with this?

04 May 2014 200 5 View

Can anyone help with the expression of androgen receptor in triple negative breast carcinoma cell line?

I have transfected androgen receptor in a triple negative breast cancer cell line. Normally, the androgen is expressed in cytoplasm and after treatment with DHT it translocates to nucleus. But...

04 May 2014 4,524 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View