I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the tissues, preserve and take them to the lab (8 hours drive from the collection point) for RNA extraction?
I have used a RNA preservation solution for lichen samples and it worked well, at least for the bacterial 16S rRNA. The product was called stayRNA but the original is known as RNAlater. There is a recipe you can find online which requires EDTA, NaOH, Sodium citrate and Ammonium sulfate. Link to users guide: https://www.aabiot.com/en/download?code=637dca71645faf17feca0256a04246856032e81e
Best of luck!
Bacterial 16S rRNA above and in seq perspective is most probably not RNA, its DNA.
Further, for the preservation of samples for RNA (not DNA) extraction, there are many products available in market. You can search for RNAlater or alternatives. If you are interested in DNA, no special solution is needed. any buffer, or even water is just fine.
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