Sheerien Rajput

11 Questions 21 Answers 0 Followers

Questions related from Sheerien Rajput

How can we define tumor micro environment?

I am interested to know the exact definition of tumor micro-environment in reference to FFPEs (Formalin Fixed Paraffin Embedded tissues) and in vivo both. Does it depend upon the physical distance...

10 October 2017 3,420 6 View

Serum starvation for synchronization of cells?

We synchronize cells for different experiments, but my question is in reference to drug treatment. If we synchronize cells by minimal or no media, should we add the treatment or drug in complete...

07 July 2017 1,678 5 View

How to calculate the molarity of a solution if we accidentally mix two aliquots of solution with different molarity ?

Hi all, I have accidentally mixed 500ul of 60mM in 1000 ul of 6mM of the same solution. The 6mM solution was diluted from the stock (60mM) with serum free media. Now I don't want to discard it...

03 March 2017 5,676 3 View

Can any one suggest the recipe of Acidified Ethanol and what percent of it is acceptable in tissue culture?

I need to dilute a drug Melphalan in acidified ethanol. The insert says3 drops of HCL in ethanol. Is it really fine to add without measuring 3 drops. Moreover I have found that Ethanol is...

03 March 2017 2,913 0 View

Any idea of a good CD28 antibody?

I need a CD28 antibody preferably Mouse/Rabbit ANTI HUMAN. I want to use it with IHC or ICC. But kindly suggest the one working with Myeloma Cells. Any help would be appreciated.

12 December 2016 4,865 6 View

Can we store cells at 4 Degree aftre fixation with 4% PFA? and can they be usd further for cytospin, IHC staining or IF?

Hi All, I wanted to know if suspension cells can be stored at 4 degrees after fixation with 4% PFA for 20 minutes. After good washing if I store them for around a week at 4 in PBS, would it have...

03 March 2016 6,531 5 View

Is there any way to select Lentiviral transduced cells other than FACS sorting?

I am using pSicoR (Plasmid #11579) from Add gene. I just wanted to know whether there is any other way , any drug resistance or something that FACS sorting can be avoided? As per the map from...

12 December 2015 9,375 1 View

Invasion Assay not working for myeloma cell lines. Could it be the pore size or chemo attractant?

I am trying Matrigel invasion assay for myeloma cell lines. I am using 8 microns membrane and coating the matrigel at 1: 3 conc. But am not getting results. I am using U266 cell line which is...

10 October 2014 3,780 4 View

What is the best method for characterization of HEK 293T Cells?

I have frozen vials of HEK 293T cells. Before using it in my experiments, I want to characterize it. Normally we can go for STR analysis but I want to do something less expensive yet standard such...

09 September 2014 7,740 4 View

Can anyone help with the expression of androgen receptor in triple negative breast carcinoma cell line?

I have transfected androgen receptor in a triple negative breast cancer cell line. Normally, the androgen is expressed in cytoplasm and after treatment with DHT it translocates to nucleus. But...

05 May 2014 4,596 3 View

Can anyone give me some information on scoring methods for IHC slides in myeloma?

I am staining myeloma samples for the expression of a panel of markers. When it comes to evaluate these slides, I cannot find any exact criteria defined in papers. Can anyone please help me with this?

05 May 2014 267 5 View