Hi All,

I wanted to know if suspension cells can be stored at 4 degrees after fixation with 4% PFA for 20 minutes. After good washing if I store them for around a week at 4 in PBS, would it have any impact on cytospin, IF or IHC. 

OR 

should they be processed in eppendorf for complete staining and then stored at 4 before cytospin.

Any help would be appreciated.

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