What is the problem with these tissue culture plants?

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Raghad Mouhamad

Nutrient Deficiency:Nitrogen Deficiency: Yellowing, especially of older leaves, is a common symptom of nitrogen deficiency. Ensure that your culture medium contains the correct concentration of nutrients. Iron Deficiency: Yellowing of younger leaves while the veins remain green can indicate iron deficiency.

Light Conditions:Ensure the plants are receiving the appropriate amount and quality of light. Too much or too little light can cause stress, leading to yellowing.

pH Imbalance:The pH of the culture medium can affect nutrient availability. Check the pH to ensure it is within the optimal range for your plant species.

Contamination:Fungal or bacterial contamination can cause yellowing. Inspect the plants and the medium for signs of contamination.

Temperature Stress:Extreme temperatures, either too high or too low, can cause chlorosis. Ensure that the temperature is stable and within the optimal range for your plants.

Watering Issues:Overwatering or underwatering can cause stress and yellowing. Ensure that the moisture levels in the culture medium are appropriate.

Toxicity:Accumulation of salts or other toxic substances in the culture medium can lead to yellowing. Regularly change the medium and use high-quality water.

Genetic Factors:Some plants may have genetic predispositions to yellowing, especially in tissue culture where somaclonal variation can occur.

Maryam Karimi Alavijeh

when do you subculture them? it seems they weren't subculture for a long time!!

Donwière Some

Je pense que les plantes qui jaunissent ont duré dans le milieu de culture. Si ce n'est pas le cas, il faut revoir la qualité de ton milieu MS. En somme ça doit être un problème de carences, soit la température de la chambre de culture est élevée.

Steven Thomas Tumaini

Dear Team

Thank you for the question, depending on jars and room temperature, TC handling needs much care at each stage. Temperature and germination issues affect the quality. Trace the handling and hygiene in your laboratory. You can go ahead and screen the source.