I am staining myeloma samples for the expression of a panel of markers. When it comes to evaluate these slides, I cannot find any exact criteria defined in papers. Can anyone please help me with this?
Usually in most of the cases of neoplastic plasma cell proliferative diseases, the
plasma cells bear morphologic resemblance to their benign mature counterparts.
The cellular morphology in such cases could be assessed on the basis of the H&E section moderately differentiated (anaplastic) sections or according to the degree of resemblance to mature plasma cells. It might also depend on the nuclear morphology and the proportion of the predominant cell type. Multiple myelomacould be studied by demonstration of light chain restriction in the cells. Cytoplasmic kappa light chain and IgG are the most common light chain and immunoglobulin classes which are present.
Other ways to characterize multiple myeloma is by - lytic bone lesions, increased numbers of clonal plasma cells within the bone marrow and by the presence of paraprotein band which could be found out in serum or urine.
Thanks for your valuable comments Bin Xu. My panel contains 6 markers localized at all the three locations. Nuclear, cytoplasmic and membranous. What I wanted to know was the exact criteria because I was getting confused with different approaches used in literature. Now I can search and get precise answer. Thanks.
Image J (with plugins for Area Calculation) is a great (and free) program that I use for quantifying fluorescent cells and tissues on slides - I see no reason my it wouldn't work for IHC as well. Apologies if you've already covered this - it's quite a long thread! Please reply iF you need more details!