I have frozen vials of HEK 293T cells. Before using it in my experiments, I want to characterize it. Normally we can go for STR analysis but I want to do something less expensive yet standard such as PCR markers for HEK 293T cells or IHC for markers.
For characterization of cancer cell, LDH assay, analysis of tumor markers by Flowcytometry and expression of p53 can be use. They might be less expensive than other.
293T cells contain the SV40 T-antigen, so you can probably have some level of confirmation based on that by PCR.
Mark's idea is the best and cheapest. If your confusion is in between HEK293 parental versus HEK293T, you can also expose them to G418/neomycin. 293T cells are resistant since they carry a neo resistant marker but parental cells are susceptible. You will need to grow cells in the presence of the drug about 3-5 days. 293 cells will die.
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