Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte drug, its possible to quantify the total amount of drug by hplc in that compartment?
Abdelhak Maghchiche
HPLC can quantify the total drug content in the opposite compartment of a transwell assay, including both free and nanoparticle-encapsulated drugs. However, this method won't distinguish between the two forms without additional steps. Proper sample preparation, potentially involving nanoparticle disruption, and careful method development are crucial for accurate quantification, especially considering the presence of nanoparticle materials in the sample.
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