I want to generate a custom polyclonal antibody for a peptide and I wanted to ask for the best company/companies based on personal experience. I'm looking for a reliable rabbit polyclonal antibody with high affinity and specificity, suitable for western, IHC, IP, and IF. Any recommendations based on experience?
First, you should make a careful decision regarding the peptide conjugate. An incorrect or unsuitable structure will lead to the failure of the project (most frequent reason).
1. Check the conjugation using MALDI.
2. Use low immunization doses for antibodies with high affinity.
3. Choose long immunization schedules with an interval of at least 4 weeks.
4. Check the antibody titer after each immunization boost in your lab.
5. Request good documentation.
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