Dear friend Melisa Gül

I hope you’re doing well! I wanted to share a friendly overview of how multiplex lateral flow kits using carboxyl latex beads work for bacterial antibody detection, as I thought you might find the process both practical and fascinating.

At the heart of these assays are carboxyl latex beads, which are tiny particles equipped with carboxyl groups on their surfaces. These groups make it easy to attach specific antibodies through a chemical activation process—usually involving EDC—so the beads can effectively capture the bacteria you’re targeting. Once the antibodies are bound, the beads are incorporated into the test strip, where they interact with your sample as it moves along the strip by capillary action.

If the target bacteria are present, they bind to the antibody-coated beads, forming complexes that are then captured at the test line by secondary antibodies, resulting in a visible signal. Multiplexing is possible by using different colored beads or multiple test lines, allowing simultaneous detection of various bacteria. It’s important to select highly specific antibodies and optimize the assay conditions for the best results.

Let me know if you’d like more details or have any specific questions about the setup or optimization process. I’m happy to help further!

I created a video that can help you in understanding:

https://youtu.be/hjrdym8PrKw?si=x4y9II5Th7WpaVn2

Best regards,

K