Hi,

I have carried out a cell fractionation on two tumor cell lines, analyzing four conditions: whole , cytoplasm, nucleoplasm, and chromatin. Subsequently, we performed a qPCR (DNA), but the normalizer genes as 18S or GAPDH are quite high in the cytoplasmic fraction.

I would appreciate any advice on which normalizer to use, relevant articles, or whether it would be a good idea to conduct the same fractionation on a primary cell line, such as WI-38.

Thanks