Hello, please can someone guide me on what to do when the purified Newcastle disease virus got bacterial contamination?. Is there a way we can simply remove the contamination from the purified virus?. Thank you for your help.
Thank you for the inform Ali. Yes, I filtered the virus immediately after purification using 0.45-micrometer filter. I realized the contamination only after when I treated the cells with the virus, while the control cells(untreated with virus) were all normal (with no contamination). Which indicated that the contamination came from the virus, not from either media or other things.
Just use a conventional bacterial filter. The pore size is about 220 nm. It is an usual practice in virology. The other way is differential centrifugation.
The contamination could be not from the virus suspension. It is possible to contaminate the cells just with manipulations. Clean the laminar box, it can be contaminated too. In additional, you can check the virus suspension with optical or electron microscopy. Sometimes we use TEM with negative staining for virus suspensions check.
Good luck!
You can use 0.2 micrometer filter. NDV can pass thru the filter. I think that's enough.
Good Luck!
Dear Denis, thank you for your helpful comments. I will act accordingly and see the outcome. Yes, Velizar, I thought of using 0.2um filter but I am not sure whether it can still be in contamination. I will try and filter it and re-infect the cells again and see what will happened next. Thank you so much.
Dear Umar, you can use some antibiotics too. Sometimes it is possible to use them at concentration lethal for microbes, but almost nontoxic for the infecting cells.
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