My question is whether or not the sequence for a Phage capsid scaffolding protein (GPO) could be used use for the production of coat proteins. I know the scaffolding protein (gpo), is required for the assembly of correctly formed viral capsids and that the capsid encloses the genetic material of the virus but what I don't know is if the capsid scaffolding protein (gpo) contains any of that genetic material or not. I am having trouble finding a capsid or coat protein sequence for a virus that I am working with but I did come across a sequence number for the Phage capsid scaffolding protein (GPO) but I need to know if I will be able to successfully make and purify coat proteins from it or not. I have a feeling the answer is no but I still need to ask.
Also, if a capsid or coat protein sequence can not be found can somebody tell me what other protein can be used to make coat proteins from (polyprotein, movement, hypothetical, heat-shock, hydrophobic, etc....)? I am fairly new in the world of proteins so if this is a ridiculous question please forgive me, thanks.
What do you want to produce? VLPs or recombinant capsid protein?
For the latter You need a capsid sequence for producing capsid proteins. For VLPs it really depends on the virus and it is not always possible. Check literature first. Its difficult to reply because there are literally billions of different phages out there and we don't know which one you are talking about.
it's true that phages of the caudovirales order use scaffolding proteins, but they have nothing to do with the viral genome. Once preformed capsids are assembled, the genome is pumped inside through a porous complex. The scaffold will be degraded by viral proteases and make way for the genome.
In any case, I suggest you spend the next two weeks just reading on basic phage biology, it will help you a lot.
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Virology