Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

ELISPOT normalization with varying cell numbers?

Hello everyone, due to a calculation error in the spreadsheet, the splenocytes I used were wrongly diluted. As a result, the number of cells per well are identical for each mouse, but differ...

15 December 2020 9,344 1 View

How do I structure my panel data in an event study?

Hey, I am conducting an event study with a short event window (lets say 3 or 5 days). I have a lot of variables, but most of them are fixed over that short period of time (GDP per capita, GDP...

11 November 2020 3,582 6 View

Jamovi - mean centred moderators?

Jamovi free ware has a built-in interaction function (a product term) in linear regression procedure. Does anybody know if Jamovi automatically mean centres variables included in the product...

04 August 2020 6,418 8 View

Supplier for thick block of brass CuZn20?

For a ballistic test I am looking for a supplier of brass CuZn20 in thick plate or billet shape We need 150 mm in total thickness with sides (or diameter of 160 mm).

17 May 2020 449 4 View

How to extract legal speed limit from GPS coordinates in OSM (OpenStreetMap) ?

I am interested in the extration of speed limit from Longitude and Latitude coordinate values. Is it possible to find speed limit attribute assigned to specific Open Street Map position and is...

30 March 2020 9,201 3 View

Human Isogenic H3.3K27M cell lines?

Hello all, I'm trying to identify pairs of isogenic human cell lines (preferably brain) in which the parent line does not possess the H3.3K27M mutation while the other one does. I've been finding...

26 March 2020 1,439 2 View

Has GPS detected the ethrer bwind?

Abstract An illusion of undecidability of an ether wind was the basis for establishing the theory of special relativity (SRT). The reason to that mistake was a wrong interpretation of light...

08 February 2020 6,990 4 View

Does source code language matter in a paper?

During literature review, I have noticed that many papers under the umbrella of software engineering use Java when code examples or small demonstrations are appropriate. I understand that Java is...

10 January 2020 1,763 7 View

How do we measure the concentration of two different complex in the same solution: Rh2(SO4)3 and RhCl3?

Hi all. I have solution containing mixture of two complexes of rhodium Rh2(SO4)3 and RhCl3, the concentration about 100 ppb - 2 ppm each with matrix SO4 2- and Cl- about 2 M, is there any...

11 November 2019 5,753 1 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View