12 May 2017 3 552 Report

I want to use influenza A virus rescued from plasmids to infect MDCK cells. Could polybrene can improve the efficiency of the infection of influenza A virus?

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Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?

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Are there any good simple systems or platforms to recommend?

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PBMC infection with virus?

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Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

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Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

How to removal of dead cells and debris from organoid cultures?

I'm currently working with human pancreatic organoids and I am wondering to remove the dead cells and debris from health organoids, any suggestions would be appreciated!

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The complex interactions between oral microbiota and oral mucosal immunoregulation: implications for oral health and disease development?

This research question explores the complex interactions between oral microbes and oral mucosal immune regulation. The diversity and function of oral microbes have important impacts on oral health...

09 July 2024 7,883 0 View

Cp of Turbine is ever increasing with Tip Speed Ratio. What Could be the reason??

I am a student of Mechanical Engineering. I am doing CFD opf Archimedes Winf Turbine. I am trying to validate the result of Paper. But the problem is that tha value of Power Coefficient is ever...

05 July 2024 6,839 2 View

How to solve the problem that the film obtained by magnetron sputtering on the ternary alloy target A2BC is ABC (not A2BC)?

Using magnetron sputtering with an alloy target composed of A:B:C in a 2:1:1 ratio, the resulting thin film composition is A:B:C=1:1:1. How can you achieve a A:B:C=2:1:1 composition? What are some...

24 June 2024 1,605 0 View

A small question about the principle of lasers…?

Given the energy level diagram of a ruby laser shown in the right figure, where \( R_{02} \) is the pumping rate, and \( \tau_2 \) and \( \tau_1 \) are the lifetimes of levels \( n_2 \) and \( n_1...

23 June 2024 4,855 0 View

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How to improve my plaque assay for influenza virus?

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